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Abstrak (Bhs. Indonesia)

Streptomyces merupakan bakteri Gram positif Familia Streptomycetaceae yang membentuk spora dan banyak ditemukan pada tanah yang alami. Streptomyces sangat menarik perhatian para ahli bakteriologi karena kemampuannya dalam mensintesis metabolit sekunder berupa senyawa antimikroba atau antibiotik. Streptomyces sp. SAC4536 merupakan actinomycetes yang terbukti mampu menghambat pertumbuhan Candida albicans. Jamur C. albicans bersifat patogen dan menyebabkan infeksi jamur yang disebut kandidiasis yaitu penyakit pada selaput lendir, mulut, vagina dan saluran pencernaan. Zat antijamur yang dihasilkan oleh Streptomyces sp. SAC4536 belum diketahui golongan senyawanya dan mekanisme antibiosisnya terhadap C. albicans. Penelitian ini bertujuan untuk mengetahui golongan senyawa antijamur yang dihasilkan oleh Streptomyces sp. SAC4536 berdasarkan metode kromatografi lapis tipis (KLT) dan mengetahui mekanisme penghambatan zat antijamur yang dihasilkan oleh Streptomyces sp.  SAC4536 terhadap C. albicans melalui deteksi kebocoran sel. Penelitian dilakukan di Laboratorium Mikrobiologi, Laboratorium Fisiologi Tumbuhan dan Laboratorium Struktur Perkembangan Hewan Fakultas Biologi Universitas Jenderal Soedirman dengan menggunakan metode survey dan analisis secara deskriptif. Penelitian ini dilaksanakan dalam waktu 8 bulan dari bulan Januari 2021 sampai Agustus 2021. Variabel yang diamati, yaitu isolat Streptomyces sp. SAC4536 dan karakteristik senyawa antijamur Streptomyces sp. SAC4536. Parameter yang diamati, yaitu golongan senyawa antijamur, nilai Rf, nilai Optical Density (OD), Minimum Inhibitory Concentration (MIC) dan diameter zona hambat. Streptomyces sp. SAC4536 memproduksi senyawa metabolit golongan senyawa flavonoid. Senyawa antijamur yang dihasilkan oleh Streptomyces sp. SAC4536 dapat menghambat C. albicans dengan mekanisme penghambatan melalui kebocoran sel, C. albicans mengalami kebocoran asam nukleat dan protein pada dinding selnya.

Abtrak (Bhs. Inggris)

Streptomyces is a Gram positive bacteria of the Streptomycetaceae family that forms spores and is commonly found in natural soils. Streptomyces has attracted the attention of bacteriologists because of its ability to synthesize secondary metabolites in the form of antimicrobial or antibiotic compounds. Streptomyces sp. SAC4536 is an actinomycetes which is proven to be able to inhibit the growth of Candida albicans. The fungus C. albicans is pathogenic and causes a fungal infection called candidiasis, which is a disease of the mucous membranes, mouth, vagina and digestive tract.  The antifungal substance produced by Streptomyces sp. SAC4536 is not yet known the class of compounds and the mechanism of their antibiosis against C. albicans. This study aims to determine the class of antifungal compounds produced by Streptomyces sp. SAC4536 based on thin layer chromatography (TLC) method and knowing the mechanism of inhibition of antifungal substances produced by Streptomyces sp. SAC4536 against C. albicans via cell leak detection. The research was conducted at the Microbiology Laboratory, Plant Physiology Laboratory and Animal Development Structure Laboratory of the Faculty of Biology at Jenderal Sudirman University using survey methods and descriptive analysis. This research was carried out within 8 months from January 2021 to August 2021. The observed variables, namely the isolates of Streptomyces sp. SAC4536 and characteristics of antifungal compounds Streptomyces sp. SAC4536. The parameters observed were antifungal compound group, Rf value, Optical Density (OD) value, Minimum Inhibitory Concentration (MIC) and the diameter of the inhibition zone. Streptomyces sp. SAC4536 produces metabolites of the flavonoid group of compounds. The antifungal compound produced by Streptomyces sp. SAC4536 can inhibit C. albicans with an inhibitory mechanism through cell leakage, C. albicans has leakage of nucleic acids and proteins in its cell wall.

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