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Nabilla Gina Irwanti

NIM

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Abstrak (Bhs. Indonesia)

Familia Cyprinidae dapat ditemukan di hampir seluruh sungai Indonesia termasuk Sungai Banjaran, Kabupaten Banyumas. Diferensial leukosit bertujuan mengetahui perbedaan persentase sel leukosit. Penelitian ini untuk mengetahui hubungan antara lokasi pengambilan sampel (hulu, tengah, dan hilir) dengan diferensial leukosit dan mengetahui gambaran apusan darah dari beberapa spesies ikan familia Cyprinidae. Pengambilan sampel menggunakan teknik purposive sampling pada tiga stasiun yang berbeda. Data yang diperoleh dianalisis dengan metode Analysis of Variance (ANOVA) dengan tingkat kesalahan 5% dan gambaran hasil pengamatan mikroskopis jenis-jenis leukosit. Hasil penelitian menunjukkan bahwa perbandingan persentase sel lekosit pada Ikan Nilem (Osteochilus vittatus), Ikan Brek (Barbonymus balleroides), dan Ikan Lunjar (Rasbora argyrotaenia) dengan lokasi Hulu, Tengah, Hilir Sungai Banjaran, Banyumas, berbeda secara signifikan (p<0,05). Persentase sel leukosit yang paling tinggi ada pada zona Hilir di Sungai Banjaran, Banyumas yaitu sel neutrofil dan sel monosit. Presentase sel neutrofil Ikan Nilem didapatkan sebesar 9,5%, Ikan Brek sebesar 8,5% dan Ikan Lunjar sebesar 9,4%. Presentase sel monosit pada Ikan Nilem sebesar 8,9%, Ikan Brek sebesar 8,7% dan Ikan Lunjar sebesar 8,5%. Sel neutrofil memiliki sitoplasma bergranula halus dan ditengahnya terdapat inti bersegmen. Sel eosinofil mempunyai sitoplasma berwarna lebih merah, inti yang terletak memanjang di tepi sel, memiliki granula besar. Sel limfosit memiliki sitoplasma berwarna biru gelap dan inti berbentuk bulat hingga oval. Sel monosit memiliki sitoplasma berwarna biru pucat dan bentuk inti bervariasi ada yang berbentuk seperti ginjal hingga yang berbentuk dua lobus besar.

Abtrak (Bhs. Inggris)

The family Cyprinidae can be found in almost all Indonesian rivers, including the Banjaran River, Banyumas Regency. Leukocyte differential aims to determine the difference in the percentage of leukocytes. This study was to determine the relationship between sampling locations (upstream, middle, and downstream) with differential leukocytes and to determine the picture of blood smears from several species of fish in the family Cyprinidae. Sampling using purposive sampling technique at three different stations. The data obtained were analyzed using the Analysis of Variance (ANOVA) method with an error rate of 5% and a description of the results of microscopic observations of the types of leukocytes. The results showed that the comparison of the percentage of leukocytes in Nilem Fish (Osteochilus vittatus), Brek Fish (Barbonymus balleroides), and Lunjar Fish (Rasbora argyrotaenia) with locations Upstream, Middle, Downstream of the Banjaran River, Banyumas, was significantly different (p<0, 05). The highest percentage of leukocyte cells is in the Downstream zone in the Banjaran River, Banyumas, namely neutrophil cells and monocyte cells. The percentage of neutrophil cells for Nilem Fish was 9.5%, Brek Fish 8.5%, and Lunjar Fish 9.4%. The percentage of monocyte cells in Nilem Fish was 8.9%, Brek Fish 8.7%, and Lunjar Fish 8.5%. Neutrophil cells have a finely granular cytoplasm and a segmented nucleus in the center. Eosinophil cells have a redder cytoplasm, the nucleus is located elongated at the edge of the cell, has large granules. Lymphocyte have a dark blue cytoplasm, and a round to oval nucleus. Monocytes have pale blue cytoplasm and the shape of the nucleus varies from kidney-shaped to two large lobes.

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