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M. SAEPUL UYUN

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Abstrak (Bhs. Indonesia)

M. SAEPUL UYUN. Universitas Jenderal Soedirman Purwokerto, Agustus 2021. Pengaruh Level dimethylacetamide (DMA) pada Pengencer Susu Skim terhadap Motilitas dan Fertilitas Spermatozoa Ayam Kampung.
Pembimbing Utama : Prof. Ir. Dadang Mulyadi Saleh, M.S., M.Agr.Sc.Ph.D.
Penelitian ini dilaksanakan di di Experimental Farm, HRJ Casiwan farm dan Laboratorium Fisiologi dan Reproduksi Ternak Terapan, Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto pada tanggal 10 Januari-20 Juni 2021.
Penelitian ini bertujuan untuk mengetahui pengaruh level dimethylacetamide (DMA) pada pengencer susu skim terhadap motilitas dan fertilitas spermatozoa ayam kampung.  Bahan yang digunakan dalam penelitian ini adalah spermatozoa segar dari 10 ekor ayam kampung umur 1-1,5 tahun dan 40 ekor ayam niaga petelur, umur 42 minggu, kuning telur ayam niaga petelur, DMA, susu skim dan aquabidest. Penelitian ini terdiri dari 2 rancangan percobaan yaitu Rancangan Acak Kelompok (RAK) untuk motilitas dan Rancangan Acak Lengkap (RAL) untuk fertilitas dengan 4 jenis perlakuan dan 5 ulangan. Perlakuan yang diberikan adalah P0: 0,5 ml spermatozoa + 0,75 ml pengencer (susu skim + kuning telur) 100%; P1: 0,5 ml spermatozoa + 0,75 ml pengencer (95% (susu skim + kuning telur) + 5% DMA); P2: 0,5 ml spermatozoa + 0,75ml pengencer (90% (susu skim + kuning telur) + 10% DMA); P3: 0,5 ml spermatozoa + 0,75 ml (85% (susu skim + kuning telur) + 15% DMA). Hasil Penelitian menunjukkan bahwa penambahan level dimethylacetamide (DMA) berpengaruh sangat nyata (P<0,01) terhadap motilitas spermatozoa ayam kampung.  Rataan hasil penelitian motilitas dari semua perlakuan adalah 74,25±15,54 persen dengan kisaran 40 persen sampai 86 persen, tetapi berpengaruh tidak nyata terhadap fertilitas, P>0,05. Disimpulkan bahwa penambahan level dimethylacetamide (DMA) 5% menghasilkan tingkat motilitas terbaik dengan nilai 80,00 ± 6.11 persen, namun angka fertilitas relatif sama.

Abtrak (Bhs. Inggris)

M. SAEPUL UYUN. Jenderal Soedirman University Purwokerto. August 2021. The Effect of dimethylacetamide (DMA) Level in Skim Milk Diluents on The Motility and Fertility of Kampung Rooster Spermatozoa.
Main Supervisor: Prof. Ir. Dadang Mulyadi Saleh, M.S., M.Agr.Sc. Ph.D.
This research was conducted at the experimental farm, HRJ Casiwan farm and the Laboratory of applied animal physiology and reproduction, Faculty of Animal Science, Jenderal Soedirman University Purwokerto from January 10 to June 20, 2021. 
The purpose of this study was to determine the effect of addition level of dimethylacetamide (DMA) in skim milk diluents on the motility and fertility of Kampung rooster spermatozoa. The materials used in this research were fresh sperm from 10 Kampung roosters with age ranging from 1-1.5 years old and 40 commercial laying hens, aging 42 weeks old, egg yolks of commercial laying hens, DMA, skim milk and aquabidest. This study consisted of 2 experimental designs, a randomized block design (RBD) for motility and a complete randomized design (CRD) for fertility with 4 types of treatments and 5 replications. The treatments given were P0 = 0.5 ml sperm + 0.75 ml diluent (skim milk + egg yolk) 100%; P1: 0.5 ml sperm + 0.75 ml diluent (95% (skim milk + egg yolk) + 5% DMA; P2 = 0.5 ml sperm + 0.75 ml diluent (90% (skim milk + egg yolk) + 10% DMA; and P3 = 0.5 sperm + 0.75 ml diluent (85% (skim milk + egg yolk) + 15% DMA). The results showed that the addition of various level of dimethylacetamide (DMA) had a very significant effect (P <0.01) on the spermatozoa motility of Kampung roosters. Average this result of research motility from all of treatment is 74.25±15.54 percent with range 40 until 86 percent, but not on the fertility, P>0.05. It can be concluded that the addition level of 5% dimethylacetamide (DMA) resulted in the best motility rate with 80.00 ± 6.11 percent.  However, the fertility rate was relatively the same.

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