Update Artikelilmiah: 32671

AULIA NURY FAZA

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Pendahuluan: Obesitas ditandai dengan ukuran lingkar pinggang lebih dari 90cm untuk laki-laki dan 80cm untuk perempuan. Salah satu faktor yang mempengaruhi obesitas adalah faktor genetik, diantaranya gen uncoupling protein (UCP)-2 Ala55Val. Latihan fisik continuous training  (CT) dapat meningkatkan perubahan ukuran lingkar pinggang dan persentase lemak tubuh pada orang obesitas, namun pengaruh polimorfisme gen UCP-2 Ala55Val terhadap perubahan lingkar pinggang pasca intervensi CT masih belum jelas.  Tujuan: Mengetahui pengaruh variasi genetik UCP-2 Ala55Val terhadap perubahan lingkar pinggang pasca intervensi continuous training pada wanita dengan obesitas. Metode: Penelitian ini menggunakan rancangan penelitian Quasi Experimental Pre and Post Design tanpa kontrol. Subjek sebanyak 14 orang yang diambil melalui purposive sampling. Pemeriksaan lingkar pinggang dilakukan sebelum dan sesudah intervensi, sedangkan polimorfisme gen UCP-2 Ala55Val sebelum intervensi. Pemeriksaan gen UCP-2 Ala55Val menggunakan metode Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR – RFLP). Data dianalisis secara univariat dan bivariat. Analisis bivariat menggunakan uji t berpasangan dan uji t tidak berpasangan. Hasil: Hasil menunjukkan lingkar pinggang sebelum intervensi sebesar 86,82±9,14cm dan setelah intervensi sebesar 82,64±7,73cm, yang dinyatakan bermakna dengan uji t berpasangan (p=0,000). Perubahan lingkar pinggang setelah intervensi pada genotip CC sebesar 3,59±3,08cm dan genotip TT 6,33±4,16cm, dinyatakan tidak bermakna dengan uji t tidak berpasangan (p=0,224). Kesimpulan: Terdapat pengaruh continuous training (CT) terhadap lingkar pinggang. Tidak ditemukan perbedaan perubahan lingkar pinggang yang bermakna pada kedua kelompok genotip polimorfisme gen UCP-2 Ala55Val.

Abtrak (Bhs. Inggris)

Introduction: Obesity is characterized by waist circumference more than 90cm for men and 80cm for women. One of the factors that influence obesity is genetic factors, including uncoupling protein (UCP)-2 Ala55Val gene polymorphism. Continuous training (CT) exercise can increase change on waist circumference and body fat percentage in obese individuals, but the impact of the UCP-2 Ala55Val gene polymorphism on waist circumference change after CT intervention is unclear. Objective: To determine the impact of UCP-2 Ala55Val genetic variation on waist circumference change post continuous training intervention in obese women.  Methods: This study used a Quasi Experimental Pre and Post Design Without Control Group design. Subjects were 14 people chosen based on purposive sampling. Waist circumference was examined before and after intervention, while UCP-2 Ala55Val gene polymorphysm before intervention. Gene examination used Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR–RFLP) method. Data were analyzed by univariate and bivariate test. Bivariate analysis was using dependent t-test and independent t-test. Results: The results indicate that waist circumference before intervention is 786,82±9,14cm and after intervention is  82,64±7,73cm, which is significant using dependent t-test (p=0,000). Waist circumference change post intervention in CC genotype is 3,59±3,08cm and TT genotype is 6,33±4,16cm, which is not significant using independent t-test (p=0,224). Conclusion: There is an effect of continuous training (CT) on waist circumference. There is no significant difference in waist circumference changes in the two groups of genotypes of the UCP-2 Ala55Val gene polymorphism.

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