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ALIFIA AYU PURWANTO PUTRI

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui interaksi antara suhu thawing dan bangsa sapi yang berbeda untuk menjaga motilitas dan viabilitas serta menekan angka abnormalitas pada spermatozoa post-thawing. Rancangan penelitian yang digunakan yaitu Rancangan Acak Lengkap (RAL) pola faktorial. Hasil analisis variansi menunjukkan faktor A (suhu thawing) memberikan pengaruh sangat nyata terhadap (P<0,01) terhadap motilitas dan viabilitas spermatozoa post-thawing. Hasil analisis variansi menunjukkan faktor B (bangsa sapi) tidak memberikan pengaruh nyata (P>0,05) terhadap motilitas dan abnormalitas sedangkan pada viabilitas memberikan pengaruh sangat nyata (P<0,01) spermatozoa post-thawing. Hasil analisis variansi menunjukkan interaksi faktor A (suhu thawing) dan B (bangsa sapi) berpengaruh tidak nyata (P>0,05) terhadap motilitas, abnormalitas, dan viabilitas. Kesimpulan penelitian ini menunjukkan bahwa tidak terdapat interaksi antar faktor perlakuan A (suhu thawing) dan perlakuan B (bangsa sapi) terhadap persentase motilitas, abnormalitas, dan viabilitas. Suhu thawing paling optimal dicapai pada suhu 37oC selama 30 detik, dengan hasil persentase motilitas dan viabilitas tertinggi dan persentase abnormalitas terendah. Bangsa sapi memberikan respon yang sama terhadap motilitas dan abnormalitas sedangkan pada viabilitas dihasilkan respon yang berbeda terhadap spermatozoa post thawing.

Abtrak (Bhs. Inggris)

The Purpose of research to knows interaction between thawing temperature and different breeds of cattle to maintain motility, viability and reduce abnormality in post-freezing sperm. The research design used was a completely randomized design (CRD) with a factorial pattern. The results of the analysis of variance showed that the A factor (thawing temperature) had a very significant effect on (P<0.01) on the motility and viability of post-thawing spermatozoa. The results of the analysis of variance showed that the B factor (bovine breed) did not have a significant effect (P>0.05) on motility and abnormalities, while on viability it had a very significant effect (P<0.01) on post-thawing spermatozoa. The results of the analysis of variance showed that the interaction of factors A (thawing temperature) and B (cow breed) had no significant effect (P>0.05) on motility, abnormality, and viability. The conclusion of this study showed that there was no interaction between treatment factors A (thawing temperature) and treatment B (cattle breed) on the percentage of motility, abnormality, and viability. The most optimal thawing temperature was achieved at 37⁰C for 30 seconds, with the highest percentage of motility and viability and the lowest percentage of abnormality. Cattle breeds gave the same response to motility and abnormalities, while the viability produced a different response to post-thawing spermatozoa.

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