Update Artikelilmiah: 32626

SEPTINA NUR AINI

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Abstrak (Bhs. Indonesia)

Fase inflamasi merupakan salah satu fase penting dalam penyembuhan luka insisi gingiva di mana terdapat peran sitokin antiinflamasi berupa IL-10 sebagai regulator utama dalam menekan inflamasi. Carica pubescens telah dilaporkan mengandung flavonoid sebagai antiinflamasi sehingga berpotensi menjadi bahan alternatif penyembuhan luka insisi gingiva. Tujuan penelitian ini untuk mengetahui pengaruh pemberian topikal gel ekstrak etanol daun Carica pubescens terhadap kadar IL-10 pada penyembuhan luka insisi gingiva tikus galur Wistar. Jenis penelitian eksperimental laboratoris in vivo dengan rancangan posttest-only control group design. Penelitian menggunakan 30 ekor tikus galur wistar jantan yang dibagi menjadi kelompok perlakuan gel ekstrak etanol daun carica konsentrasi 12,5%, 25%, 50%, kontrol positif Aloclair gel, dan kontrol negatif CMC-Na. Pengambilan sampel jaringan gingiva dilakukan pada hari ke-3. Hasil pemeriksaan kadar IL-10 dengan metode ELISA pada kelompok gel ekstrak etanol konsentrasi 12,5%, 25%, 50%, kontrol positif Aloclair gel, kontrol negatif CMC Na berturut-turut ialah 60,77 ± 5,59; 57,73 ± 2,66; 56,02 ± 3,01; 59,88 ± 1,69; 41,07 ± 3,80 pg/mL. Kadar IL-10 paling tinggi pada pemberian gel ekstrak konsentrasi 12,5%. Hasil analisis statistik menunjukkan terdapat perbedaan yang signifikan (p<0,05) antar kelompok perlakuan dengan kontrol negatif namun tidak terdapat perbedaan signifikan (p>0,05) dengan kontrol positif. Kesimpulan dari penelitian ini adalah terdapat pengaruh pemberian gel ekstrak etanol daun carica terhadap peningkatan kadar IL-10 pada proses penyembuhan luka insisi gingiva tikus galur wistar dengan konsentrasi gel paling berpengaruh 12,5%.

Abtrak (Bhs. Inggris)

Inflammatory phase is one of the important phases in gingival incision wounds healing process, where there is a role for antiinflammaatory cytokines such as IL-10 as the main regulator in suppressing inflammation. Carica pubescens has been reported contain flavonoid as an antiinflammation therefore potential as an alternative for post-incision gingival wound healing. The objective of this study was to determine the effect of topical application of Carica pubescens leaf ethanolic extract gel on IL-10 level in gingiva incision wound healing process in wistar strain rat. In vivo laboratory experimental technique was used in this study with posttest-only control group design. This study used 30 male wistar rats which were divided into treatment groups treated with carica leaf ethanol extract gel with concentration of 12.5%, 25%, 50%, also Aloclair gel positive control group and CMC-Na negative control group. Gingival tissue samples were collected on the third day. The result of analyzed IL-10 levels using ELISA method for Carica pubescens leaf ethanolic extract gel treated groups with 12,5%, 25%, 50% concentration, Aloclair gel positive control and CMC-Na negative control groups were 60,77 ± 5,59; 57,73 ± 2,66; 56,02 ± 3,01; 59,88 ± 1,69; 41,07 ± 3,80 pg/mL, respectively. The IL-10 levels were decrease as the carica leaf ethanol extract gel concentration increase. The highest IL-10 concentration was found in the treatment group with 12,5% extract concentration. Statistical analysis result showed a significant difference (p <0,05) between treatment groups and negative control but there was no significant difference (p> 0,05) with positive control group. The conclusion of this study is there was an effect of carica leaf ethanolic extract gel toward increasing level of IL-10 in post-incision gingival wound healing in wistar rat with the most effective concentration is 12,5%.

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