Update Artikelilmiah: 32608

SABRINA AULIA NURUL HIDAYAH

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Abstrak (Bhs. Indonesia)

Luka insisi gingiva menimbulkan kerusakan struktur anatomi gingiva. Fase awal penyembuhan luka yang sangat penting adalah fase inflamasi yang melibatkan sitokin proinflamasi TNF-α. TNF-α meningkat 6-24 jam pasca luka, apabila tidak segera menurun maka menyebabkan inflamasi yang berkepanjangan. Daun Carica pubescens diketahui mengandung flavonoid yang memiliki efek antiinflamasi. Tujuan penelitian ini adalah mengetahui pengaruh pemberian topikal gel ekstrak etanol daun Carica pubescens terhadap kadar TNF-α pada penyembuhan luka insisi gingiva tikus galur Wistar. Jenis penelitian ini adalah eksperimental laboratoris in vivo dengan posttest-only control group design. Sampel berjumlah 30 tikus galur Wistar jantan, dibagi menjadi 5 kelompok yaitu kelompok perlakuan gel ekstrak etanol daun Carica pubescens konsentrasi 12,5%, 25%, 50%, kontrol positif Aloclair gel, dan kontrol negatif CMC-Na 5%. Pengambilan sampel jaringan gingiva dilakukan hari ke-3 pasca luka insisi dan pemberian perlakuan untuk dilakukan pemeriksaan kadar TNF-α dengan metode ELISA. Hasil pengukuran kadar TNF-α pada kelompok gel esktrak etanol daun Carica pubescens 12,25%, 50%, kontrol positif Aloclair gel, dan kontrol negatif CMC-Na 5% secara berturut-turut adalah 86,44 ± 26,70, 98,63 ± 36,58, 101,58 ± 33,43, 88,74 ± 27,08, 179,46 ± 85,88 pg/ml. Hasil penelitian menunjukkan bahwa pemberian gel ekstrak etanol daun Carica pubescens menurunkan kadar TNF-α signifikan (p<0,05) dibandingkan kontrol negatif dan tidak terdapat perbedaan pengaruh yang signifikan (p>0,05) dengan kontrol positif. Pemberian gel ekstrak etanol daun Carica pubescens konsentrasi 12,5% menurunkan TNF-α yang paling baik. Kesimpulan penelitian ini adalah terdapat pengaruh pemberian gel ekstrak etanol daun Carica pubescens terhadap kadar TNF-α pada proses penyembuhan luka insisi gingiva tikus galur Wistar.

Abtrak (Bhs. Inggris)

Gingival wound from incision procedure causes damage to the anatomical structure of the gingiva. Inflammation phase is a very important in initial wound healing process that involve the proinflammatory cytokines such as TNF-α. TNF-α level increases in 6-24 hours after trauma and if doesn’t immediately decrease will cause prolonged inflammation. Carica pubescens leaves contain flavonoid compound which is known to have antiinflammatory effect. The objective of this study was to determine the effect of Carica pubescens leaf ethanolic extract gel as a topical application on TNF-α level in incision gingiva wound Wistar rats healing process. The study was in vivo experimental laboratory research with posttest-only control group design. Thirty male Wistar rats were divided into 5 groups: treatment groups of Carica pubescens leaf ethanol extract gel with concentration of 12.5%, 25%, 50% respectively, positive control group treated with Aloclair gel, and negative control group treated with CMC-Na 5%. Gingiva tissue samples were collected in the third day after incision and treatment followed with TNF-α level measurement using ELISA method. The results of the measurement of TNF- levels in the treatment group of Carica pubescens leaf ethanol extract gel with concentration of 12.5%, 25%, 50%, positive control group treated with Aloclair gel, and negative control group treated with CMC-Na 5% were 86.44 ± 26.70, 98.63 ± 36.58, 101.58 ± 33.43, 88.74 ± 27.08, 179.46 ± 85.88 pg/ml respectively. The results showed that the application of Carica pubescens leaf ethanol extract gel reduced the levels of TNF-a significantly (p<0.05) compared to the negative control and there was no significant difference (p>0.05) with the positive control group. The application of Carica pubescens leaf ethanol extract gel with a concentration of 12,5% had the best result in reducing TNF-a levels. The conclusion of this study is there was an effect of topical application of Carica pubescens leafs ethanolic extract gel on TNF-α level in incision gingival wound Wistar rats healing process.

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