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MUHAMMAD ARIEF ABYAN SHIDIQ

NIM

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui medium cair yang tepat untuk perbanyakan jamur patogen gulma, dan 2) virulensi jamur patogen hasil perbanyakan dari medium alternatif terbaik terhadap gulma. Penelitian ini dilaksanakan di Laboratorium Perlindungan Tanaman dan screen house, Fakultas Pertanian, Universitas Jenderal Soedirman dari Agustus 2020 hingga November 2020. Penelitian ini terdiri atas dua tahap, penelitian tahap I adalah perbanyakan jamur patogen gulma pada medium cair alternatif, dengan rancangan acak lengkap faktorial, dan faktor yang diuji jenis jamur patogen gulma (Chaetomium sp., Fusarium oxysporum, Curvularia sp.) dan jenis medium cair (PDB, air cucian beras, limbah cair tahu). Variabel yang diamati kerapatan konidium jamur patogen gulma. Penelitian tahap II adalah uji virulensi jamur patogen terhadap gulma in-vivo, dengan rancangan petak terbagi, dan faktor yang diuji jenis jamur patogen (Chaetomium sp., F. oxysporum, Curvularia sp.) dan jenis gulma (rumput kenop, rumput grinting, krokot, dan babadotan). Variabel yang diamati masa inkubasi, intensitas penyakit, laju infeksi, AUDPC, tinggi tanaman, jumlah daun, bobot tajuk basah, bobot tajuk kering, bobot akar basah, dan bobot akar kering. Hasil penelitian menunjukkan bahwa medium air cucian beras efektif digunakan sebagai medium alternatif perbanyakan jamur patogen gulma. Pada perlakuan medium air cucian beras, jumlah konidium jamur lebih tinggi 68,1% dibanding medium PDB. Sedangkan jumlah konidium pada medium limbah cair tahu lebih tinggi 40,6% dibanding medium PDB. Jamur Chaetomium sp. dan Curvularia sp. mampu menyebabkan penyakit serta menghambat pertumbuhan gulma dengan masa inkubasi lebih cepat 36 dan 33%, intensitas penyakit lebih besar 77,7 dan 74,2%, AUDPC lebih besar 80,8 dan 79,9% dibanding kontrol. Jumlah daun lebih rendah 31,6 dan 29,8%, bobot tajuk kering lebih rendah 36,1 dan 29,7% dibanding kontrol, sedangkan pada tinggi tanaman, bobot tajuk basah, bobot akar basah dan kering tidak berbeda nyata.

Abtrak (Bhs. Inggris)

The research aimed to determine the right liquid media for propagation of weed pathogenic fungi, and the virulence of the pathogenic fungi from the best alternative medium to weeds. This research was conducted at the Plant Protection Laboratory and the screen house, Faculty of Agriculture, Jenderal Soedirman University, from August 2020 to November 2020. This research consisted of two stages, the first stage was multiplication of weed pathogenic fungi in alternative liquid medium, used a factorial completely randomized design, with the factors tested were the pathogenic fungi (Chaetomium sp., F. oxysporum, Curvularia sp.) and the liquid media (PDB, rice washing water, tofu liquid waste). Variables observed were conidia density. The second stage was to test the virulence of pathogenic fungi against weeds in vivo, used a splitplot design with the pathogenic fungi (Chaetomium sp., F. oxysporum, Curvularia sp.) as main plot and the weeds (knop grass, grinting grass, purslane, and babadotan) as subplot. Variables observed were incubation period, disease intensity, infection rate, AUDPC, plant height, number of leaves, shoot wet and dry weight, and root wet and dry weight. The results showed that the rice washing water medium was effectively used as an alternative medium for the propagation of weed pathogenic fungi. In the rice washing water medium, the number of mushroom conidiums was 68.1% higher than the PDB medium. Meanwhile, the amount of conidium in tofu liquid waste medium was 40.6% higher than PDB medium. Chaetomium sp. and Curvularia sp. capable of causing disease and inhibiting weed growth with an incubation period of 36 and 33% faster, greater disease intensity of 77.7 and 74.2%, AUDPC greater than 80.8 and 79.9% compared to controls. The number of leaves was 31.6 and 29.8% lower, the dry crown weight was 36.1 and 29.7% lower than the control, while the plant height, wet shoot weight, wet and dry root weights were not significantly different.

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