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TANIA RIZKIANI

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Abstrak (Bhs. Indonesia)

Penelitian bertujuan untuk 1) mengisolasi bakteri endofit daun padi yang berpotensi sebagai agens pengendali R.solani, 2) mengidentifikasi bakteri endofit daun padi yang dapat menghambat pertumbuhan R.solani penyebab penyakit hawar pelapah padi, dan (3) mengetahui karakterisasi bakteri endofit daun padi yang berpotensi sebagai agens pengendali hayati. Penelitian dilaksanakan pada bulan Desember 2019 - September 2020 di Laboratorium Perlindungan Tanaman dan Screen House Fakultas Pertanian Universitas Jenderal Soedirman. Sampel tanaman padi diambil dari pertanaman padi di Kecamatan Purwokerto Utara. Sampel daun padi untuk isolasi bakteri endofit diambil di lahan tanaman padi di 3 kecamatan yaitu Kutasari, Padamara dan Patikraja. Kecamatan Patikraja diambil dari 2 desa pada lahan marginal yaitu Notog dan Kedung Randu. Pengambilan sampel menggunakan metode purposive random sampling. Variabel yang diamati adalah persentase daya hambat, masa inkubasi, intensitas penyakit, laju infeksi, tinggi tanaman, jumlah anakan, bobot segar tanaman, bobot segar akar, volume akar, bobot kering tanaman dan bobot kering akar. Hasil penelitian menunjukkan bahwa isolasi bakteri endofit daun padi menghasilkan 11 isolat. Isolat bakteri umumnya memiliki bentuk bulat dan hasil pewarnaan Gram keseluruhan berwarna ungu yang menunjukkan bakteri Gram positif umumnya tidak memiliki endospora. Isolat bakteri endofit terpilih yaitu KDS 6, MDP 1, dan NDP memiliki rata-rata daya hambat terhadap R.solani secara berturut-turut adalah 11,65%, 38,33%, dan 31,68%. Isolat bakteri endofit terpilih yaitu KDS 6, MDP 1, dan NDP secara berturut-turut memiliki masa inkubasi 22 HSI, 7 HSI, dan 19 HSI. Isolat bakteri ini dapat menekan pertumbuhan R.solani tetapi tidak berpengaruh dalam memacu pertumbuhan tanaman padi.

Abtrak (Bhs. Inggris)

This study aims to 1) isolate the rice leaf endophytic bacteria which have the potential to control R.solani, 2) identify the rice leaf endophytic bacteria which can inhibit the growth of R.solani which causes sheath blight, and (3) determine the characterization of rice leaves endophytic bacteria  which has the potential as a biological control agent.The research was carried out in December 2019 - September 2020 at the Plant Protection Laboratory and Screen House of the Faculty of Agriculture, Jenderal Soedirman University. Rice samples were taken from rice fields in North Purwokerto District. Rice leaves samples for the isolation of endophytic bacteria were taken from rice fields in 3 districts, namely Kutasari, Padamara and Patikraja. Patikraja sub-district was taken from 2 villages on marginal land, namely Notog and Kedung Randu. Sampling using purposive random sampling method. The variables observed were the percentage of inhibition, incubation period, disease intensity, infection rate, plant height, number of tillers, plant fresh weight, root fresh weight, root volume, plant dry weight and root dry weight. The results showed that the isolation of rice leaves endophytic bacteria resulted in 11 isolates. Bacterial isolates generally have a round shape and overall Gram stain is purple which indicates Gram positive bacteria generally do not have endospores. The selected endophytic bacterial isolates, namely KDS 6, MDP 1, and NDP had an average inhibitory power against R.solani, respectively, 11.65%, 38.33%, and 31.68%. The selected endophytic bacterial isolates had an incubation period of KDS 6 22 HSI, MDP 1 7 HSI, and NDP 19 HSI. These bacterial isolates can suppress the growth of R.solani but have no effect in spurring the growth of rice.

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