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WINARDO FRANSISKUS

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian minyak safflower (Carthamus tinctorius L) dan inositol serta campuran minyak safflower dan inositol terhadap jumlah leukosit dan diferensial leukosit darah ayam Sentul jantan. Materi yang digunakan dalam penelitian ini adalah ayam Sentul jantan varietas kelabu dari Balai Pengembangan Pembibitan Ternak Unggas Jatiwangi yang berumur 4 minggu berjumlah 81 ekor. Ayam Sentul jantan dipelihara selama 8 minggu dan dipanen pada umur 16 minggu. Penelitian menggunakan metode eksperimen secara in vivo dengan analisis data menggunakan model matematis Rancangan Acak Lengkap (RAL) yang terdiri dari 9 perlakuan dengan 3 kali ulangan, setiap ulangan terdiri dari 3 ekor ayam. Perbedaan antara rerata perlakuan dengan uji Beda Nyata Jujur. Perlakuan yang diuji terdiri dari R0 = PB/kontrol; R1 = PB+Minyak safflower 0,5%; R2 = PB+Minyak safflower 1,0%; R3= PB+Inositol 0,5%; R4 = PB+Inositol 1,0%; R5 = PB+Minyak safflower 0.5%  dan inositol 0,5%; R6 = PB+Minyak safflower 0.5%  dan inositol 1,0%; R7 = PB+Minyak safflower 1,0%  dan inositol 0,5%, dan R8 =PB+Minyak safflower 1,0%  dan inositol 1,0%. Hasil analisis variansi menunjukan bahwa penggunaan minyak safflower (Carthamus tinctorius L) dan inositol berpengaruh tidak nyata terhadap jumlah leukosit dan diferensial leukosit (P>0,05). Rataan jumlah leukosit yaitu antara 8,50 – 10,78.103/mm3.  Rataan persentase heterofil  yaitu R0= 35,33 ± 7,23; R1= 33,33 ± 17,01; R2= 33,00 ± 8,54; R3= 31,00  ± 6,24; R4= 41,00  ± 9,54; R5= 25,00  ± 10,82; R6= 36,67  ± 6,03; R7= 26,67  ± 8,33; R8= 31,33 ± 11,37. Rataan persentase limfosit yaitu R0= 59,00 ± 5,29; R1= 60,33 ± 20,50; R2= 59,67 ± 8,41; R3= 62,67  ± 5,03; R4= 54,00  ± 6,56; R5= 67,00  ± 12,12; R6= 56,00  ± 2,65; R7= 67,00  ± 7,94; R8= 63,67 ± 13,80. Rataan persentase monosit R0= 5,67 ± 2,52; R1= 5,67 ± 3,79; R2= 5,33 ± 2,08; R3= 5,33  ± 1,15; R4= 5,00  ± 3,00; R5= 6,67  ± 3,06; R6= 7,33  ± 3,51; R7= 6,33  ± 3,51; R8= 5,00 ± 2,65. Disimpulkan bahwa penggunaan minyak safflower dan inositol tidak memberikan pengaruh negatif terhadap jumlah leukosit dan diferensial leukosit darah ayam Sentul jantan karena berada pada persentase normal.

Abtrak (Bhs. Inggris)

The research was aimed to evaluate the effect of safflower oil (Carthamus tinctorius L) and inositol as well as a mixture of safflower oil and inositol to Leukocyte Number and Differential Leukocyte in Sentul chicken. The material used in this research is kelabu variety Sentul chicken from Balai Pengembangan Pembibitan Ternak Unggas Jatiwangi, 4 week old chickens, totaling 81 birds. The Sentul roosters will be maintained for 4 weeks so that they will be harvested at 12 weeks. The research used an in vivo with data analysis using mathematical metode  a Completely Randomized Design (CRD) consisting of 9 treatments with 3 replications, each replication consisted of 3 chickens. The treatments that have significant effect will be followed by the Honestly Significant Difference test. The treatments consisted of R0 = Basal feed/ control; R1 = Basal feed + Safflower oil 0,5%; R2 = Basal feed + Safflower oil 1,0%; R3= Basal feed + Inositol 0,5%; R4 = Basal feed + Inositol 1,0%; R5 = Basal feed + Safflower oil 0.5%  and inositol 0,5%; R6 = Basal feed + Safflower oil 0.5% and inositol 1,0%; R7 = Basal feed + Safflower oil 1,0%  and inositol 0,5%, dan R8 = Basal feed + Safflower oil 1,0%  and inositol 1,0%.The results of analysis of variance showed that the used safflower oil and inositol non significant to Leukocyte Number and Differential Leukocyte (P>0,01). The average leukocyte number is between 8,50 – 10,78 x 103/mm3. The average percentage heterophil R0= 35,33 ± 7,23; R1= 33,33 ± 17,01; R2= 33,00 ± 8,54; R3= 31,00  ± 6,24; R4= 41,00  ± 9,54; R5= 25,00  ± 10,82; R6= 36,67  ± 6,03; R7= 26,67  ± 8,33; R8= 31,33 ± 11,37. The average percentage limfosit R0= 59,00 ± 5,29; R1= 60,33 ± 20,50; R2= 59,67 ± 8,41; R3= 62,67  ± 5,03; R4= 54,00  ± 6,56; R5= 67,00  ± 12,12; R6= 56,00  ± 2,65; R7= 67,00  ± 7,94; R8= 63,67 ± 13,80. The average percentage monosit R0= 5,67 ± 2,52; R1= 5,67 ± 3,79; R2= 5,33 ± 2,08; R3= 5,33  ± 1,15; R4= 5,00  ± 3,00; R5= 6,67  ± 3,06; R6= 7,33  ± 3,51; R7= 6,33  ± 3,51; R8= 5,00 ± 2,65. The use of safflower oil and inositol had no negative effect on the number of leukocytes and differential leukocytes in Sentul chicken blood because it was at a percentage that normal.

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