Update Artikelilmiah: 28644

HASSAN ISHAG HASSAN HAREN

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Judul Artikel

Abstrak (Bhs. Indonesia)

The present study aimed to investigate the association between myostatin (MSTN) genotype and two traits, growth and carcass, in Batur sheep. Blood samples
representing thirty head were collected and genomic DNA was extracted. A specific primer designed to amplify the MSTN gene, samples sequenced then, used the BioEdit program to identify any mutation. Calculation of genotypes, gene and allele frequencies,
heterozygosities, and Chi-square test was performed. Only two alleles observed (G and C) resulted in three genotypes. 11 polymorphic sites were observed, transversion at c.*121G>C, and one individual G>A which disrupted reading frame of whole MSTN sequenced, genotypic and allelic frequencies were 0.552 GG, 0.379 GC, and 0.069 CC, where the allele frequency was 0.741 G and 0.259 C. Lambs that carrying genotype GC had slightly more pre-slaughter weight, hot carcass weight, cold carcass weight, carcass length, and total yield (lion, shoulder, leg yield, and thigh) compared to those carrying genotype GG. There was no significant effect of the MSTN genotype on carcass traits (p=0.05). Polymorphic site c.*121 G>C is present in Batur sheep for the first time about the association with the MSTN gene however, it has not effects weaning weight, 6-month weight, and studied carcass traits

Abtrak (Bhs. Inggris)

The present study aimed
to investigate the association between myostatin (MSTN) genotype and two traits, growth and carcass, in Batur sheep. Blood samples
representing thirty head were collected and genomic DNA was extracted. A specific primer designed to amplify the MSTN gene,
samples sequenced then, used the BioEdit program to identify any mutation. Calculation of genotypes, gene and allele frequencies,
heterozygosities, and Chi-square test was performed. Only two alleles observed (G and C) resulted in three genotypes. 11 polymorphic
sites were observed, transversion at c.*121G>C, and one individual G>A which disrupted reading frame of whole MSTN sequenced,
genotypic and allelic frequencies were 0.552 GG, 0.379 GC, and 0.069 CC, where the allele frequency was 0.741 G and 0.259 C. Lambs
that carrying genotype GC had slightly more pre-slaughter weight, hot carcass weight, cold carcass weight, carcass length, and total
yield (lion, shoulder, leg yield, and thigh) compared to those carrying genotype GG. There was no significant effect of the MSTN
genotype on carcass traits (p=0.05). Polymorphic site c.*121 G>C is present in Batur sheep for the first time about the association with
the MSTN gene however, it has not effects weaning weight, 6-month weight, and studied carcass traits.

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