Update Artikelilmiah: 25408

INDRA PANGESTU

NIM

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Abstrak (Bhs. Indonesia)

Produk utama tanaman kelapa sawit adalah minyak kelapa sawit. Produksi tanaman kelapa sawit Indonesia masih rendah yakni baru mencapai 23,53 juta ton atau masih berkisar antara 3-4 ton tandan buah segar (TBS)/ha per tahun. Asam lemak rantai panjang disintesis oleh enzim asetil-KoA karboksilase (ACCase), berbagai hasil penelitian menunjukkan adanya korelasi antara aktivitas ACCase dan kecepatan sintesis asam lemak maupun dengan akumulasi minyak Penelitian ini bertujuan untuk mengetahui pengaruh teknik pengendapan protein menggunakan amonium sulfat (NH4SO4) terhadap kualitas enzim asetil-KoA karboksilase serta mengetahui pengaruh penambahan aktivator terhadap aktivitas enzim asetil-KoA karboksilase. Penelitian dilaksanakan di Laboratorium Biokimia dan Biologi Molekuler Pusat Penelitian Bioteknologi dan Bioindustri Indonesia Bogor pada bulan Oktober 2018 sampai dengan April 2019. Penelitian ini menggunakan metode ekstraksi protein dengan tris HCl, EDTA dan β-merkaptoetanol sebagai bahan pelarut. Sampel yang diperoleh kemudian ditambahkan amonium sulfat (NH4SO4) dan dilakukan dialisis. Tahap selanjutnya adalah pengujian profil protein menggunakan SDS PAGE untuk sampel crude extract, crude extract + dialisis dan crude extract + amonium sulfat + dialisis, kemudian dilakukan kromatografi filtrasi gel sephadex G-25 yang dilanjutkan dengan pengujian konsentrasi protein menggunakan uji bradford. Pengukuran aktivitas enzim asetil-KoA dilakukan menggunakan HPLC (High Performance Liquid Chromatography) untuk sampel crude extract, dialisis dan sampel hasil kromatografi filtrasi gel sephadex G-25 dan dilanjutkan dengan pengukuran aktivitas enzim asetil-KoA karboksilase dengan penambahan beberapa konsentrasi asam glutamat. Variabel yang diamati adalah konsentrasi protein, profil protein dan juga aktivitas enzim asetil-KoA karboksilase. Hasil penelitian ini menujukkan bahwa pengendapan dengan amonium sulfat menyebabkan protein menjadi jenuh, hasil terbaik untuk variabel pengukuran protein pada SDS PAGE adalah sampel crude extract + dialisis. Konsentrasi protein yang didapat dari hasil kromatografi filtrasi gel sephadex G-25 yang terbesar sebanyak 11,543 mg/ml. Pengukuran aktivitas enzim asetil-KoA tertinggi didapatkan pada sampel kromatografi filtrasi gel sebesar 46,034 gram per liter asetil per menit . Penambahan asam glutamat mampu meningkatkan aktivitas enzim asetil-KoA sampai 2x lipat dengan aktivitas tertinggi sebesar 19,702 gram per liter asetil per menit  dengan konsentrasi optimum penambahan sebsar 20 ppm.

Abtrak (Bhs. Inggris)

The main product of oil palm plants is palm oil. Indonesia is the top occupies position in achieving the area and production of world palm oil which reaches 8.9 million hectares with 6.5 million hectares in the form of producing plants (TM). The production of oil palm plants from the area of the plantations is still low, reaching only 23.53 million tons or still around 3-4 tons of fresh fruit bunches (TBS) / ha per year. Long chain fatty acids are synthesized by the acetyl-CoA carboxylase (ACCase) enzyme, various research results indicate a correlation between the activity between ACCase and the speed of fatty acid synthesis as well as oil accumulation. This study aims to determine the effect of protein deposition techniques using ammonium sulfate the quality of the enzyme acetyl-CoA carboxylase and knowing the effect of adding activators to the activity of the enzyme acetyl-CoA carboxylase. The research was conducted at the Laboratory of Biochemistry and Molecular Biology, Bogor Indonesia Biotechnology and Bioindustry Research Center in October 2018 to April 2019. This study used a protein extraction method with tris HCl, EDTA and β-mercaptoetanol as solvents. The samples obtained were then added ammonium sulfate (NH4SO4) and dialysis. The next stage is protein profile testing using SDS PAGE for crude extract samples, crude extract + dialysis and crude extract + ammonium sulfate + dialysis. The next step is sephadex G-25 gel filtration chromatography followed by protein concentration testing using the Bradford test. Measurement of acetyl-CoA enzyme activity was carried out using HPLC (High Performance Liquid Chromatography) for crude extract, dialysis samples and sephadex G-25 gel filtration chromatography samples and continued by measuring the activity of the enzyme acetyl-CoA carboxylase by adding several concentrations of glutamic acid. The variables observed were protein concentration, protein profile and also the activity of the enzyme acetyl-CoA carboxylase. The results of this study are that precipitation with ammonium sulfate causes protein to become saturated, the best result for the protein measurement variable in SDS PAGE is a sample of crude extract + dialysis. The greatest concentration of protein obtained from sephadex G-25 gel filtration chromatography was 11,543 mg / ml. The highest measurement of acetyl-CoA enzyme activity was obtained in gel filtration chromatography samples of 46,034 gram per liter acetyl per minute . The addition of glutamic acid was able to increase the activity of the acetyl-CoA enzyme to 2x with the highest activity of 19.702 gram per liter acetyl per minute   with optimum concentration of addition of 20 ppm glutamic acid.

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