Update Artikelilmiah: 25012

RESTA FAUZI TRI ANGGESARA

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk; 1) mengetahui jenis tepung yang tepat untuk perbanyakan Metarhizium anisopliae, 2) mengetahui pengaruh media perbanyakan Metarhizium anisopliae dari beberapa jenis tepung terhadap mortalitas dan intensitas serangan ulat crop (Crocidolomia pavonana F.), 3) mengetahui pengaruh Metarhizium anisopliae terhadap bobot segar tanaman pakcoy. Penelitian dilaksanakan di Laboratorium Ilmu Hama Tumbuhan dan Screen house Fakultas Pertanian Universitas Jenderal Soedirman, Purwokerto, pada bulan Oktober 2018 sampai Januari 2019. Penelitian dilakukan melalui dua tahap yaitu in vitro dan in planta. Penelitian tahap pertama (in vitro) menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan,  yaitu media perbanyakan PDB (P0), tepung kedelai 2% (P1), tepung terigu 2% (P2), tepung beras 1,5% (P3), dan tepung ketan 1,5% (P4). Penelitian tahap kedua (in planta) menggunakan Rancangan Acak Kelompok (RAK) dengan 8 perlakuan dan 4 ulangan, setiap ulangan terdapat 2 unit tanaman, perlakuan yang diujikan yaitu kontrol negatif (K-), kontrol positif (K+), media perbanyakan PDB (P0), tepung kedelai 2% (P1), tepung terigu 2% (P2), tepung beras 1,5% (P3), tepung ketan 1,5% (P4), pestisida decis (P5). Variabel pengamatan pada tahap pertama yaitu kerapatan spora, serta tahap kedua meliputi mortalitas larva, intensitas serangan, dan bobot segar tanaman. Hasil penelitian menunjukan bahwa 1) tepung kedelai 2% merupakan media perbanyakan M. anisopliae terbaik dengan jumlah kerapatan spora sebesar 3,08 x 107 spora/ml, 2) tepung kedelai 2% mampu menimbulkan mortalitas C. pavonana sebesar 28,75%, 3) pemberian M. anisopliae tidak berpengaruh terhadap intensitas serangan hama dan bobot segar tanaman.

Abtrak (Bhs. Inggris)

This research was aimed to; 1) knowing the type of flour that is right for the propagation of Metarhizium anisopliae, 2) knowing the effect of the propagation  media of Metarhizium anisopliae from several types of flour on the mortality and intensity of cobbage caterpillar attack (Crocidolomia pavonana F.), 3) knowing the effect of Metarhizium anisopliae on the fresh weight of pakcoy plants. This research was conducted in Plant Pest Science Laboratory of Fakulty of Agriculture, Jenderal Soedirman University, Purwokerto, from Oktober 2018 to Januari 2019. The research was conducted through two stages, that is in vitro and in planta. The first stage (in vitro) of the study used a completely randomized design (CRD) with 5 treatments and 5 replications, that is propagation media of PDB (P0), 2% soybean flour (P1), 2% wheat flour (P2), 1,5% rice flour (P3), 1,5% glutinous flour (P4). The second stage (in planta) of the study used a Randomized Block Design (RBD) with 8 treatments and 4 replications, each replication has 2 units of plants, the treatments tested were negative control (K-), positive control (K +), propagation media of PDB (P0), 2% soybean flour (P1), 2% wheat flour (P2), 1.5% rice flour (P3), glutinous flour 1.5% (P4), decis pesticide (P5). The observation variable in the first stage was spore density, and the second stage included the larval mortality, intensity of attack, and fresh weight of plants. The results showed that 1) 2% soybean flour is the best M. anisopliae propagation medium with a total spore density of 3.08 x 107 spores / ml, 2) 2% soybean flour can cause a mortality of 28.75%, 3) the application M. anisopliae didn’t have affect the intensity of pest attacks and the fresh weight of plants.

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