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WIWIN MASRIA YOSEFINE SIHOTANG

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui penggunaan bahan beras ketan sebagai formula cair Trichoderma harzainum serta konsentrasi yang efektif untuk mengendalikan penyakit rebah kecambah pada mentimun. Penelitian dilaksanakan dengan metode eksperimen dalam 2 tahap, in vitro di Laboratorium Perlindungan Tanaman dan in planta di rumah plastik di Fakultas Pertanian, Universitas Jenderal Soedirman pada bulan September 2018 sampai bulan Desember 2018. Pengujian in vitro menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan, meliputi perlakuan formula cair Potato Dextrose Broth (PDB), tepung beras ketan dengan konsentrasi 5, 10, 15, dan 20 g/L. Pengujian in planta menggunakan Rancangan Acak Kelompok dengan 6 perlakuan dan 5 ulangan, meliputi kontrol dan 5 formula T. harzianum. Variabel yang diamati meliputi kepadatan konidium, masa inkubasi, kejadian penyakit, area under disease progress curve (AUDPC), tinggi tanaman, bobot segar tanaman, panjang akar, bobot segar akar, jumlah daun, potensi tumbuh maksimum dan daya kecambah. Hasil penelitian menunjukkan bahwa pemformulaan tepung beras ketan dapat digunakan sebagai medium alternatif pertumbuhan T. harzianum. Kepadatan konidium T. harzianum tertinggi pada formula medium cair tepung beras ketan dengan konsentrasi 20 g/L, yaitu sebanyak 4,80 X 107 konidium/mL, dan efektif menekan penyakit rebah semai 88,89% dan menunda masa inkubasi selama 7 hari bila dibandingkan dengan kontrol. Perlakuan tersebut juga memiliki nilai AUDPC terkecil sebesar 17,5. Pemformulaan tepung beras ketan juga berpengaruh pada komponen pertumbuhan, salah satunya berpengaruh terhadap panjang tajuk sebesar 13,80% dibanding kontrol.

Abtrak (Bhs. Inggris)

This research aimed at knowing the use of glutinous rice flour as liquid formulation of Trichoderma harzainum and its effective concentration to control damping off in cucumbers. This research was carried out at the Plant Protection Laboratory and the screen house area, the Faculty of Agriculture, Jenderal Sudirman University from September 2018 up to Desember 2018. In vitro test used a completely randomized design with 5 treatments and 5 replicates, consisted of Potato Dextrose Broth (PDB), glutinous rice flour with a concentration of 5, 10, 15, and 20 g/L. The in planta test used a randomized block design with 6 treatments and 5 replications, included controls and 5 formulas of T. harzianum. Variables observed included conidia density, incubation period, disease incidence, area under disease progress curve (AUDPC), plant height, plant fresh weight, root length, root fresh weight, number of leaves, maximum growth potential, and germination. Results of the research showed that the formula of glutinous rice flour can be used as an alternative medium for growth of T. harzianum. The highest conidia density of T. harzianum was found at glutinous rice flour formula with a concentration of 20 g/L, which was as much as 4.80 X 107 conidia/mL, and effective to suppress the incidence of disease to 88,89% and delay the incubation period for 7 days compared to controls. This treatments also have the smallest value of AUDPC as 17.5. The formulation also influenced the growth component, such as on canopy length of 13.80% compared to the control.

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