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PRODUK FERMENTASI RUMEN DOMBA YANG DIBERI RANSUM MENGANDUNG ECENG GONDOK (Eichhornia crassipes) AMONIASI DAN DIFERMENTASI MENGGUNAKAN RAGI TEMPE
Abstrak (Bhs. Indonesia)
Penelitian yang bertujuan untuk mengetahui pengaruh pemberian eceng gondok amoniasi dan difermentasi menggunakan ragi tempe terhadap produk fermentasi rumen domba, telah dilaksanakan menggunakan metode experimental invitro menurut Tilley and Terry (1963) yang dimodifikasi oleh Sutardi (1979), dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan ternak, Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto. Penelitian menggunakan rancangan acak lengkap. Perlakuan yang diuji terdiri dari empat yaitu P0 (60% Pakan konsentrat + 40% Rumput lapang), P1 (60% Pakan konsentrat + 30% Rumput lapang + 10% Eceng gondok), P2 (60% Pakan konsentrat + 30% Rumput lapang + 10% Eceng gondok amoniasi) dan P3 (60% Pakan konsentrat + 30% Rumput lapang + 10% Eceng gondok amoniasi fermentasi). Setiap perlakuan diulang lima kali, sehingga terdapat 20 unit percobaan. Peubah yang diukur adalah konsentrasi N-NH3 dan sintesis protein mikroba. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh sangat nyata (P<0,01) terhadap sintesis protein mikroba serta berpengaruh nyata (P<0,05) terhadap konsentrasi N-NH3. Hasil uji kontras orthogonal menunjukkan bahwa rataan P3 menghasilkan konsentrasi N-NH3 tertinggi sebesar 11,36 ± 0,60 mM dan sintesis protein mikroba tertinggi sebesar 175,86 ± 13,97 mg/20ml. Disimpulkan bahwa ransum yang mengandung eceng gondok amoniasi yang difermentasi menggunakan ragi tempe menghasilkan konsentrasi N-NH3 dan sintesis protein mikroba tertinggi.
Abtrak (Bhs. Inggris)
The research aimed to know the effect of ammonia and fermented water hyacinth using tempe yeast to rumen’s fermentation of sheep products, has been implemented using experimental method of invitro according to Tilley and Terry (1963) that modified by Sutardi (1979), has been undertaken at the Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto. The research using a completely randomized design. The treatments were P0 (60% concentrate feed + 40% field grass), P1 (60% concentrate feed + 30% field grass + 10% water hyacinth), P2 (60% concentrate feed + 30% field grass + 10% water hyacinth amoniation), P3 (60% concentrate feed + 30% grass + 10 % water hyacinth ammonia fermentation). Each treatment was repeated five times, so there 20 units of the experiment. The measured variables were concentration of N-NH3 and microbial protein synthesis. The results of this research showed that the effect was highly significant (P <0.01) on the microbial protein synthesis and significantly (P <0.05) on the N-NH3 concentration. The result of orthogonal contrast test showed that the average of P3 produce the highest concentration of N-NH3 of 11.36 ± 0.60 mM and the highest microbial protein synthesis of 175.86 ± 13.97 mg/20ml. It was concluded that diet containing water hyacinth amoniated and fermented using yeast tempe produce concentration of N-NH3 and microbial protein synthesis was the highest.
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