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Abstrak (Bhs. Indonesia)

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) merupakan vius
beta-coronavirus endemis di Semenanjung Arab. MERS-CoV umumnya terdeteksi pada
penderita pneumonia dengan riwayat perjalanan ke Semenanjung Arab, sehingga
penderita pneumonia dengan riwayat perjalanan ke Semenanjung Arab disebut sebagai
penderita pneumonia terduga MERS-CoV. Banyaknya agen patogen, selain MERS-CoV
yang menyebabkan pneumonia mendorong dilakukannya deteksi bakteri pada sampel
yang telah diketahui memiliki hasil negatif terhadap MERS-CoV. Tujuan dari penelitian
ini yaitu untuk mengetahui jenis bakteri yang terdeteksi serta mengetahui positivitas dari
deteksi bakteri pada kasus pneumonia terduga MERS-CoV menggunakan teknik analisis
Multiplex Real-Time RT-PCR. Penelitian ini menggunakan metode survei dengan
rancangan cross sectional. Sampel yang digunakan yaitu 38 sampel, terutama sampel
saluran pernapasan bawah berupa sputum dan bilasan Endotracheal tube (ETT), serta
sampel saluran pernapasan atas berupa swab tenggorok kasus pneumonia terduga MERSCoV yang dikirim ke Laboratorium Penelitian Penyakit Infeksi Prof. Dr. Sri Oemijati,
Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan, Jakarta.
Hasil penelitian menyatakan dari 38 sampel terdapat 25 (65,79%) sampel terdeteksi
positif mengandung satu bakteri dan 20 sampel diantaranya merupakan kasus koinfeksi. Bakteri Haemophilus influenzae (26,4%) terdeteksi dengan presentase positivitas tertinggi. Hasil penelitian ini memberikan informasi ilmiah mengenai jenis bakteri penyebab pneumonia, serta memberikan prosedur pemeriksaan yang efisien dalam mendiagnosis etiologi pneumonia yang tepat.

Abtrak (Bhs. Inggris)

Middle East Respiratory Syndrome Coronavirus (MERS-COV) is a betacoronavirus
endemic in the Arabian Peninsula. MERS-CoV commonly detected in pneumonia patients who had traveled from the middle east area. Therefore, pneumonia patients who had traveled from Arabian Peninsula usually called MERS-CoV suspected patients. There are many pathogenic agents beside MERS-CoV that caused pneumonia. So detection for those pathogenic agent must be done swiftly, especially for the negative
MERS-CoV sample. The aims of this study were to know the kind of bacterial pneumonia
that detected as pathogenic agent and positivity of each bacterial pneumonia from MERSCoV
suspected patients samples. This study designed by a descriptive research with a
cross sectional study. The samples that used in this research were upper respiratory tract
samples (throat swabs) and lower respiratory tract samples (endotracheal tube (ETT) and
sputum) from samples pneumonia suspected MERS-CoV cases which sent to the
Laboratory of Research on Infectious Diseases Prof. Dr. Sri Oemijati, Center of
Biomedics and Basic Technology of Health, Jakarta. A set of 38 samples were tested and
the results showed that 25 (65,79%) samples were positive with contained one bacteria,
including five samples were coinfection cases. The most frequent bacteria that detected
is Haemophilus influenzae with the percentage of positivity 26,4%. This results provide
an estimate of the prevalence of each bacterial pneumonia and provide the laboratory
inspection efficiently to diagnose the etiology of pneumonia.

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