Update Artikelilmiah: 21415

ELLA AULIYA NURUL BAITY

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Abstrak (Bhs. Indonesia)

Pseudomonas fluorescens merupakan salah satu bakteri antagonis karena memiliki kemampuan mengimbas ketahanan sistemik tanaman. Bakteri ini mampu menghasilkan enzim kitinase, enzim protease, dan beberapa metabolit sekunder antimikroba, asam sianida, antibiotik 2,4 diacetylphloroglucinol phenazine, pyrrolnitrin, pyoluteorin, dan biosurfactant. Potensi tersebut dapat digunakan untuk mengendalikan serangga hama. Penelitian ini bertujuan untuk mengetahui: 1) Pengaruh metabolit sekunder P. fluorescens Strain P32 terhadap mortalitas larva Helicoverpa armigera Hubner, 2) Pengaruh metabolit sekunder bakteri tersebut terhadap penghambatan aktivitas makan larva H. armigera, 3) Pengaruh metabolit sekunder bakteri tersebut terhadap pembentukan pupa H. armigera, 4) Pengaruh metabolit sekunder bakteri tersebut terhadap pemunculan imago H. armigera. 
Penelitian dilaksanakan di Laboratorium Perlindungan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman mulai bulan Februari sampai dengan September 2017. Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan 6 perlakuan dan 4 ulangan. Perlakuan terdiri dari P0 (Kontrol), P1 (1 ml/100 ml akuades), P2 (2 ml/100 ml akuades), P3 (3 ml/100 ml akuades), P4 (4 ml/100 ml akuades), dan P5 (5 ml/100 ml akuades). Variabel yang diamati yaitu mortalitas larva, bobot pakan setelah perlakuan, persentase pembentukan pupa, dan persentase pembentukan imago. Data dianalisis menggunakan uji F kemudian dilanjutkan uji DMRT pada taraf 5%. Hasil penelitian menunjukkan metabolit sekunder bakteri tersebut: 1) Mampu mematikan larva H. armigera sebesar 80%, 2) Menghambat aktivitas makan larva H. armigera sebesar 82,8%, 3) Menurunkan persentase pembentukan pupa H. armigera menjadi sebesar 20%, dan 4) Menurunkan persentase pembentukan imago H. armigera sebesar 20%.

Abtrak (Bhs. Inggris)

Pseudomonas fluorescens is an antagonistic bacteria that have ability to increase systemic defences of plants. This Bacteria produce chitinase enzyme, protease enzyme, and some secondary metabolites like antimicrobial, cyanide acid, antibiotic, 2,4 diacetylphloroglucinol phenazine, pyrrolnitrin, pyoluteorin and biosurfactant. The potential could be used to control pests. This research aimed to determine: 1) The effect of secondary metabolites from bacteria P. fluorescens strain P32 against larval mortality of Helicoverpa armigera Hubner, 2) The effect of secondary metabolites against inhibition of feeding activity of H. armigera, 3) The effect of secondary metabolites against pupal formation of H. armigera, and 4) The effect of secondary metabolites against emergence of adult H. armigera. 
This research was conducted at the Laboratory of Plant Protection, Faculty of Agriculture, Jenderal Soedirman University, from February until September 2017. The reseach used Randomized Block Design (RBD) with 6 treatments and 4 replications. Treatments consisted of P0 (control), P1 (1 ml/100 ml aquades), P2 (2 ml/100 ml aquades), P3 (3 ml/100 ml aquades), P4 (4 ml/100 ml aquades), dan P5 (5 ml/100 ml aquades). Observed variables were larval mortality, food weight after treatment, percentation of pupal formation, and percentation of H. armigera adult emerged. The data were analyzed by using the F test and followed by Duncan Multiple Range Test (DMRT). The results showed that secondary metabolites : 1) Were capable to kill larvae of H. armigera to 80%, 2) Inhibited feeding activity to 82.8%, 3) Decreased the percentage of pupal formation to 20%, and 4) Decreased the percentage of adult emerged to 20%.

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