Update Artikelilmiah: 20903

FIRDAUSA DWI ARIYANTI

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

ABSTRAK

Tuberkulosis adalah penyakit infeksi menular tertinggi di Indonesia yang disebabkan oleh kuman Mycobacterium tuberculosis. Diagnosis TB ditegakkan dengan pemeriksaan sputum secara mikroskopis.  Pemeriksaan ini prosesnya cepat, mudah, murah, tetapi angka sensitivitasnya rendah yaitu 22%.  Penelitian ini bertujuan untuk mengetahui perbandingan penemuan jumlah basil tahan asam (BTA) antara metode pemeriksaan konvensional dan pemekatan. Penelitian ini menggunakan design cross sectional, dengan jumlah sampel sebanyak 25 spesimen dari pasien TB paru dewasa di RSUD Prof. Dr. Margono Soekarjo dan BP4 Purwokerto yang memenuhi kriteria inklusi. Satu sampel dilakukan pemeriksaan BTA mikroskopis dengan metode konvensional dan metode pemekatan sentrifugasi. Jumlah BTA dihitung 100 lapang pandang dan diperiksa oleh dua pengamat. Nilai kappa antara dua pengamat yaitu 0,842. Data dianalisis menggunakan uji non-parametrik Wilcoxon dan didapatkan terdapat perbedaan yang bermakna rerata jumlah BTA per 100 lapang pandang antara metode pemeriksaan konvensional (4329,12) dan pemekatan (6298,52) dengan p=0,000. Terdapat peningkatan penemuan jumlah BTA sebesar 36% menggunakan metode pemekatan bila dibandingkan metode konvensional. Berdasarkan penelitian ini dapat disimpulkan bahwa metode pemekatan sentrifugasi dapat meningkatkan penemuan jumlah BTA pada pemeriksaan sputum mikroskopis sebesar 36%.

Abtrak (Bhs. Inggris)

THE COMPARISON OF PRESENCE ACID-FAST BACILLI (AFB) BETWEEN CONVENSIONAL AND CONCENTRATION 
METHODS EXAMINATION

Firdausa Dwi Ariyanti.1, Wahyu Siswandari.2, Indah Rahmawat..3
1Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java, Indonesia
2Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java, Indonesia
3Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java, Indonesia
Email:firdariyant@yahoo.com 
		
ABSTRACT

Tuberculosis is the highest infectious disease in Indonesia caused by Mycobacterium tuberculosis. The diagnosis of tuberculosis is established by microscopic sputum examination. This examination process is fast, easy, cheap, but it has low sensitivity about 22%. The objective of this research is to find out the comparison of presence acid-fast bacilli (AFB) between conventional and consentration methods examination. This study use design cross sectional analytic, within 25 sampels from adult patient with pulmonary tuberculosis which fulfilled the inclusion criteria in di RSUD Prof. Dr. Margono Soekarjo and BP4 Purwokerto. A sputum sample is applied by AFB examination microscopic using two method about convensional and consentration sentrifugation methods. The number AFB is calculated to 100 fields and checked by two observers. Kappa value between two obeservers is 0.842. Data was analyzed by using Wilcoxon non-parametric test and it showed there was the difference mean of the number AFB/100 fields between convensional method (4329.1) and consentration method (6298.52) about p=0.000. There was increasing mean of number AFB using consentration method 36% than convensional method. Based on this result, it can be concluded by using the consentration centrifugation method can increase the presence of number acid-fast bacilli (AFB) in sputum microscopic examination about 36%.

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