Update Artikelilmiah: 18096

NUGRO TRI WICAKSO

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Abstrak (Bhs. Indonesia)

Penelitian bertujuan mengkaji pengaruh suplementasi sulfur dan asam isobutirat serta pengaruh interaksi antara keduanya terhadap aktivitas protease cairan rumen dan kecernaan protease kasar pakan kambing. Materi yang digunakan antara lain pakan basal yang terdiri dari rumput lapang dan konsentrat (60:40), cairan rumen dari 3 ekor kambing Jawarandu yang diambil di Rumah Pemotongan Hewan (RPH) Sokaraja segera setelah kambing dipotong. Konsentrat terdiri dari dedak padi 22%, pollard 3,3%, dan bungkil kelapa 14,67%. Penelitian dilaksanakan menggunakan metode eksperimental secara in vitro dengan Rancangan Acak Lengkap (RAL) pola Faktorial 3x3 yang terdiri dari 9 perlakuan dan 3 kali ulangan. Faktor pertama (A) yaitu suplementasi sulfur yang terdiri dari 3 taraf yaitu 0% (a1), 0,3% (a2), dan 0,6 % (a3), faktor kedua (B) yaitu suplementasi asam isobutirat yang terdiri dari 3 taraf yaitu 0 mM (b1), 0,2 mM (b2), dan 0,4 mM (b3). Perlakuan yang diberikan yaitu a1b1: pakan basal + sulfur 0 % + isobutirat 0 mM, a1b2: pakan basal + sulfur 0 % + isobutirat 0,2 mM, a1b3: pakan basal + sulfur 0% + isobutirat 0,4  mM, a2b1: pakan basal + sulfur 0,3 % + isobutirat 0 mM, a2b2: pakan basal + sulfur 0,3 %+ isobutirat 0,2 mM,  a2b3: pakan basal + sulfur 0,3 % + isobutirat 0,4 mM, a3b1: pakan basal + sulfur 0,6 % + isobutirat 0 mM, a3b2: pakan basal + sulfur 0,6 % + isobutirat 0,2 mM, a3b3: pakan basal + sulfur 0,6 % + isobutirat 0,4 mM. Variabel yang diukur yaitu aktivitas protease dan kecernaan protein kasar. Data dianalisis menggunakan analisis variansi (ANAVA) dan dilanjutkan dengan uji orthogonal polynomial. Hasil penelitian menunjukkan bahwa suplementasi sulfur dan interaksi antara suplementasi sulfur dengan asam isobutirat tidak berpengaruh (P>0,05) terhadap aktivitas protease, namun demikian semakin tinggi taraf suplementasi isobutirat, aktivitas protease semakin menurun. Interaksi antara suplementasi sulfur dengan asam isobutirat berpengaruh sangat nyata (P<0,01) secara kuadrater menurunkan kecernaan protein kasar, pada titik minimum PA0 (0,38 ; 6,70) dan PA1 (0,36 ; 6,57) serta pada titik maksimum PA2 (0,21 ; 17,21). Berdasarkan hasil penelitian dapat disimpulkan bahwa semakin tinggi taraf suplementasi asam isobutirat, aktivitas protease cairan rumen semakin menurun. Semakin tinggi taraf suplementasi sulfur, kecernaan protein kasar pakan semakin meningkat.

Abtrak (Bhs. Inggris)

The aim of this research was to assess the influence of sulphur and isobutyric acid supplementation and their interaction on protease activity of rumen fluid and crude protein digestibility of goat feed. The materials used were basal feed that consisted of field grass and concentrate with a ratio of 60:40%, rumen fluid of Jawarandu goat from abattoir in Sokaraja immediately after the goat were slaughtered. The concentrate was composed of rice bran 22%, pollard 3.3%, and coconut meal 14.67%. This research was conducted by using experimental method based on Completely Randomized Design (CRD), factorial pattern (3x3) with 9 treatments and 3 repetitions. The first factor (A) was sulphur supplementation, there were 0% (a1), 0.3% (a2), and 0.6 % (a3). The second factor (B) was isobutyric acid supplementation, there were 0 mM (b1), 0.2 mM (b2), and 0.4 mM (b3).  The combination treatments given were a1b1: basal feed + sulphur 0% + isobutyric acid 0 mM, a1b2: basal feed + sulphur 0% + isobutyric acid 0.2 mM, a1b3: basal feed + sulphur 0% + isobutyric acid 0.4 mM, a2b1: basal feed + sulphur 0.3% + isobutyric acid 0 mM, a2b2: basal feed + sulphur 0.3% + isobutyric acid 0.2 mM, a2b3: basal feed + sulphur 0.3% + isobutyric acid 0.4 mM, a3b1: basal feed + sulphur 0.6% + isobutyric acid 0 mM, a3b2: basal feed + sulphur 0.6% + isobutyric acid 0.2 mM, a3b3: basal feed + sulphur 0.6% + isobutyric acid 0.4 mM. The parameters measured were protease activity of rumen fluid and crude protein digestibility. Data were analyzed using Analysis of Variance (ANOVA) advanced with orthogonal polynomial test. The results of this study showed that the supplementation of sulphur and interaction between sulphur and isobutyric acid did not significantly affect (P>0.05) protease activity, but higher isobutyric acid supplementation, protease activity became decrease further at a higher level. The interaction between sulphur and isobutyric acid supplementation highly significantly affected (P<0.01) a quadratic decrease of crude protein digestibility at a minimum point, PA0 (0.38 ; 6.70) and PA1 (0.36 ; 6.57) also at a maximum point PA2 (0.21 ; 17.21). The conclusion of this study is, higher supplementation of isobutyric acid, protease activity of rumen fluid becomes decrease at a higher level. Higher supplementation of sulphur, crude protein digestibility of feed increases at a higher level.

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