Update Artikelilmiah: 17631

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Abstrak (Bhs. Indonesia)

Daun jambu biji (Psidium guajava) memiliki kandungan senyawa flavonoid yang diketahui berkhasiat sebagai antiinflamasi dan antialergi. Tujuan dari penelitian ini adalah untuk mengetahui efek fraksi etil asetat (FEA) daun jambu biji terhadap kontraksi otot polos trakea marmut yang diinduksi metakolin.
FEA daun jambu biji diperoleh dari ekstrak etanolik daun jambu biji yang difraksinasi bertingkat menggunakan n-heksane, kloroform, dan etil asetat. Uji farmakodinamik FEA dilakukan menggunakan metode organ terisolasi. Konsentrasi FEA yang diujikan yaitu 0,05; 0,1; dan 0,2 mg/ml. Masing-masing konsentrasi diuji efeknya terhadap kontraksi otot polos pada trakea marmut yang diinduksi metakolin konsentrasi 10-8-10-3. Hasil uji berupa respon kontraksi otot polos trakea marmut yang terekam pada rekorder. Hasil tersebut diubah ke dalam persen respon kontraksi otot polos trakea marmut yang disajikan dalam bentuk kurva hubungan terhadap logaritma konsentrasi metakolin. Selanjutnya, dari persen respon kontraksi otot polos dan logaritma konsentrasi metakolin dapat dihitung nilai pD2 metakolin dengan atau tanpa penambahan FEA. Nilai pD2 metakolin dengan atau tanpa penambahan FEA dianalisis secara statistik dengan uji One-way Anova. 
Hasil penelitian menunjukkan FEA konsentrasi 0,05; 0,1; dan 0,2 mg/ml mampu menurunkan respon kontraksi otot polos trakea marmut dan mampu menggeser kurva ke arah kanan. FEA daun jambu biji konsentrasi 0,05 dan 0,2 mg/ml mampu menurunkan pD2 metakolin secara signifikan (sig<0,05). Berdasarkan hasil tersebut, dapat disimpulkan bahwa FEA daun jambu biji memiliki aktivitas sebagai antagonis reseptor asetilkolin muskarinik.

Abtrak (Bhs. Inggris)

Guava (Psidium guajava) leaves contains flavonoids that are known efficacious as anti-inflammatory and antiallergy. The aim of this study was to determine the effects of ethyl acetate fraction (FEA) of guava leaves against guinea pig tracheal smooth muscle contraction induced by methacholine.
FEA of guava leaves were obtained from fractionation of ethanolic extract by n-heksane, chloroform and ethyl acetate. FEA pharmacodynamic test was determined using an isolated organ. The concentration of FEA were 0.05; 0.1; and 0.2 mg/ml. Each concentration was tested its effect on smooth muscle contraction in guinea pig trachea induced by methacholine concentration of 10-8-10-3. The test results in the form of a response guinea pig tracheal smooth muscle contraction was recorded. The results are converted into percent response of guinea pig tracheal smooth muscle contraction which was presented in the form of curves against the logarithm of the concentration of methacholine. Furthermore, the response of the percentage of smooth muscle contraction and the logarithm methacholine concentration could be calculated the value of pD2 methacholine with or without the addition of FEA. Value pD2 methacholine with or without the addition of FEA were statistically analyzed by One-way Anova test.
The results showed that FEA concentration of 0.05; 0.1; and 0.2 mg/ml could decrease the response guinea pig tracheal smooth muscle contraction and could shift the curve to the right. There was decreasing of the value of pD2 methacholine due to the addition of FEA with concentrations of 0.05 and 0.2 mg/ml significantly (sig <0.05). Based on the results, FEA of guava leaves had antagonist activity on acethylcholine muscarinic receptors.

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