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MILA AMALIA FITRIANA

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk : 1) Mengetahui perubahan bobot tubuh larva dengan perlakuan bakteri P. fluorescens, 2) Mengetahui keragaman mikroba simbion dalam organ pencernaan larva O. rhinoceros dan H. armigera setelah perlakuan bakteri P. fluorescens. Penelitian dilaksanakan di Laboratorium Perlindungan Tanaman mulai bulan Mei sampai dengan November 2016. Rancangan yang digunakan Rancangan Acak Lengkap dengan dua perlakuan dengan tigapuluh ulangan. Perlakuan P60 dan kontrol. Variabel yang diamati yaitu perubahan bobot tubuh dan keragaman jenis mikroba simbion. Metode yang dilakukan antara lain, explorasi serangga, perbanyakan serangga, perbanyakan P60, aplikasi, pengukuran dan identifikasi. Data bobot tubuh dianalisis menggunakan uji T, dan data jenis mikroba simbion dianalisis deskriptif komparatif. Hasil penelitian menunjukkan P60 dapat menurunkan bobot tubuh Oryctes rhinoceros 10% dan Helicoverpa armigera 16%.  Perlakuan P60 dapat menurunkan keragaman mikroba simbion larva. Pada perlakuan kontrol larva Oryctes rhinoceros terdapat empat jenis mikroba simbion antara lain, Serratia marcescens, Bacillus thuringiensis, Bacillus licheniformis, Fusarium oxysforum. Pada larva Oryctes rhinoceros dengan P60 hilangnya Fusarium oxysforum. Pada larva Helicoverpa armigera tanpa perlakuan terdapat empat jenis mikroba simbion antara lain, Serratia marcescens, Bacillus amyloliquefaciens, Bacillus subtilis, Enterobacter cloaceae. Pada larva Helicoverpa armigera dengan P60 hilangnya Serratia marcescens.

Abtrak (Bhs. Inggris)

This research aimed to 1) to know changes in the larval body weight treated with the bacterium Pseudomonas fluorescens, and 2) to know diversity of symbiont microbes in the digestive organs of O. rhinoceros and H. armigera larvae after treatments with the bacterium Pseudomonas fluorescens. The research was carried out in Plant Protection Laboratory Faculty of Agriculture Jenderal Soedirman University, starting from May to November 2016. It used a complete randomized design with two treatment and 30 replicate, namely control and P60 treatment. Variables observed were changes in body weight and diversities in types of symbiont microbes. Methods done were insect exploration, insect mass production, P60 multiplication, application, measurement and identification. Data of body weight were analyzed by using T test and data of symbiont microbe diversities were analyzed using descriptively comparative analysis. Result of the research performed that P60 could reduce 10% of larval O. rhinoceros body weights and 16% of larval H. armigera body weights. Based on T that Pf P60 caused reduction in larval body weights after treatment. P60 could reduce the symbiont microbe diversities. Control on O. rhinoceros were observed to get four symbiont such as S. marcescens, B. thuringiensis, B. licheniformis, F. oxysporum. S. marcescens, B. amyloliquefaciens, B. subtilis, Enterobacter cloaceae were found on control of H. armigera larvae. P60 treatment could eliminate F. oxysforum in the O. rhinoceros digestive organs and eliminate S. marcescens in the H. armigera digestive organs.

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