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IKA TYAS AGUS PRASTIWI

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Abstrak (Bhs. Indonesia)

Aterosklerosis merupakan salah satu komplikasi tersering pada keadaan hiperkolesterolemia. Aterosklerosis meningkatkan kadar C-Reactive Protein (CRP) yang merupakan salah satu prediktor kuat kejadian penyakit kardiovaskuler. Daun kersen mengandung antioksidan, antiinflamasi dan antihiperkolesterolemia yang diharapkan menurunkan kadar CRP dalam darah. Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak etanol daun kersen (Muntingia calabura l.) terhadap kadar CRP tikus putih (Rattus norvegicus) model hiperkolesterolemia. Penelitian ini merupakan eksperimental dengan rancangan post test only with control group. Objek penelitian 26 ekor tikus putih dibagi ke dalam 5 kelompok perlakuan. Kelompok 1 (kontrol sehat, n=5), kelompok 2 (kontrol negatif, n=5), kelompok 3 (daunkersen 42mg/200grBB, n=5), kelompok 4 (daunkersen 84mg/200grBB, n=5), kelompok 5 (daunkersen 42mg/200grBB, n=6). Induksi hiperkolesterolemia pada kelompok 2, 3, 4, dan 5 menggunakan minyak babi 2 mL/200grBB/hari dan kuning telur bebek 2 mL/200gramBB/hari selama 14 hari, serta pakan buatan berbahan dasar otak sapi 10 gram/100gramBB selama 3 hari. Pemeriksaan kadar CRP serum dilakukan dengan ras Hs-CRP Elisa kit setelah pemberian ekstrak daun kersen selama 14 hari. Uji distribusi data menggunakan Saphiro-Wilk, uji beda menggunakan Kruskall wallis dan dilanjutkan uji Mann-whitney. Rerata kadar CRP kelompok 1: 278,6 ± 95,38 ng/L, kelompok 2: 382,47 ± 39,65 ng/L, kelompok 3: 313,01 ± 56,77 ng/L, kelompok 4: 274,94 ± 32,48 ng/L, dan kelompok 5: 262,30 ± 51,42 ng/L. Rerata kadar CRP kelompok 3, 4 dan 5 lebih rendah daripada kelompok 2 (p<0,05). Kesimpulan penelitian ini ekstrak etanol daun kersen dapat menurunkan kadar CRP tikus model hiperkolesterolemia dengan dosis efektif minimal 42 mg/200gramBB.

Abtrak (Bhs. Inggris)

Atherosclerosis is one of the most common complications in hypercholesterolemia. Atherosclerosis increases the level of C-Reactive Protein (CRP), which is a strong predictor for cardiovascular disease. Muntingia calabura L. leaves contain antioxidant, antiinflammation, and antihypercholesterolemia which are expected to decrease the level of CRP in blood. The purpose of this study was to know the effect of Muntingia calabura l. leaves on CRP level of hypercholesterolemia albino rat (Rattus norvegicus) models. This study used a post-test only with control group experimental study design. The objects of this study were 30 albino rats which were categorized into 5 treatment groups. The groups were as follow: group 1 (healthy control, n=5), group 2 (negative control, n=5), group 3 (Muntingia calabura L. leaves 42 mg/200gramBW, n=5), group 4 (Muntingia calabura L. leaves 84 mg/200gramBW, n=5), and group 5 (Muntingia calabura L. leaves 168 mg /200gramBW, n=6). Hypercholesterolemia induction towards group 2, 3, 4, and 5 were done by giving 2 ml/200gramBW/day of lard and 2 ml/ 200gramBW/day of duck egg yolk for 14 days, and 10 gram/100gramBW of artificial feed made of cow’s brain for 3 days. Serum CRP level test was done by using Hs-CRP ELISA kit after Muntingia calabura L. leaves extract had been given for 14 days. The tests done for the data of this study were data distribution test using Saphiro-Wilk, difference test using Kruskall wallis, and Mann-whitney test. Mean of CRP level in group 1: 278,6 ± 95,38 ng/L, group 2: 382,47 ± 39,65 ng/L, group 3: 313,01 ± 56,77 ng/L, group 4: 274,94 ± 32,48 ng/L, and group 5: 262,30 ± 51,42 ng/L. Mean of CRP levels for group 3, 4, and 5 were lower than  group 2 (p<0,05). The conclusion for this study is that Muntingia calabura L. leaves can decrease the CRP level of hypercholesterolemia albino rat models with minimal dose is 42 mg/200gramBW.

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