Update Artikelilmiah: 15151

RIDA FARHANI

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui kemampuan metabolit sekunder gabungan dua isolat Pseudomonas fluorescens dan dua isolat Trichoderma sp. dalam menekan pertumbuhan Colletotrichum musae serta pengaruhnya terhadap masa inkubasi C. musae, luas serangan C. musae, intensitas serangan C. musae, dan mutu buah pisang lepas panen. Penelitian dilaksanakan di Laboratorium Perlindungan Tanaman Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto, mulai dari Desember 2015 sampai Maret 2016. Penelitian menggunakan Rancangan Acak Lengkap pada uji in vitro dan Rancangan Acak Kelompok pada uji in vivo dengan 5 perlakuan dan lima ulangan, pada uji in vivo masing-masing unit perlakuan menggunakan 3 buah pisang. Perlakuan terdiri atas kontrol, Trichoderma sp. isolat jahe dan P. fluorescens P60, Trichoderma sp. isolat bawang merah dan P. fluorescens P60, Trichoderma sp. isolat jahe dan P. fluorescens P32, serta Trichoderma sp. isolat bawang merah dan P. fluorescens P32. Variabel pada uji in vitro yaitu tingkat penghambatan antagonis, variabel pada uji in vivo yaitu masa inkubasi, intensitas penyakit, luas serangan, serta mutu buah yang meliput kekerasan, kadar gula, dan uji organoleptik. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder Trichoderma sp. isolat jahe + metabolit sekunder P. fluorescens P60 dan metabolit sekunder Trichoderma sp. isolat jahe + metabolit sekunder P. fluorescens P32 mampu menekan pertumbuhan C. musae masing-masing sebesar 52,44% dan 48,89% in vitro, sedangkan pada uji in vivo metabolit sekunder Trichoderma sp. isolat bawang merah + metabolit sekunder P. fluorescens P32 mampu menekan luas serangan sebesar 19,26% dan intensitas serangan sebesar 15,21%. Semua perlakuan metabolit sekunder belum mampu mempertahankan mutu serta kualitas warna, rasa, aroma, tekstur, dan kesukaan buah pisang lepas panen.

Abtrak (Bhs. Inggris)

This research aimed to determine the ability of secondary metabolites combination of two Pseudomonas fluorescens isolates and two Trichoderma sp. isolates in suppressing the growth of Colletotrichum musae and their effect on incubation period of C. musae, attack area of C. musae, disease intensity of C. musae and quality of the postharvest banana. This research was conducted at the Laboratory of Plant Protection, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto, from December 2015 to March 2016. Completely Randomized Design was used in in vitro test and Randomized Block Design in in vivo test repeated five times with five plural, every unit had three bananas. The treatments were control, Trichoderma sp. ginger isolate and P. fluorescens P60 isolate, Trichoderma sp. shallot isolate and P. fluorescens P60 isolate, Trichoderma sp. ginger isolate and P. fluorescens P32 isolate, and Trichoderma sp. shallot isolate and P. fluorescens P32 isolate. Variables observed in in vitro tests were inhibition level, variables observed in in vivo tests were incubation period, attack area, disease intensity, and quality of the harvest that was harderness, sugar content, and organoleptic tests. The research result indicated that secondary metabolite of Trichoderma sp. ginger isolate + P. fluorescens P60 isolate and Trichoderma sp. ginger isolate + P. fluorescens P32 were able to inhibit the pathogen growth of C. musae in vitro respectively by 52.44% and 48.89%, while in in vivo test, secondary metabolite of Trichoderma sp. shallot isolate + P. fluorescens P32 isolate were able to suppress the attack area as 19,26% and disease intensity as 15,21%. All secondary metabolite plural have not been able to maintain the quality and color, flavor, flavour, texture, and favorite postharvest banana.

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