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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengkaji pengaruh lama thawing semen beku sapi Simmental terhadap motilitas dan intergritas membran spermatozoa. Metode penelitian yang digunakan adalah eksperimental laboratorium dengan Rancangan Acak Lengkap (RAL). Perlakuan yang diujicobakan yaitu T1= Thawing pada menit ke-15, T2= Thawing pada menit ke - 30, T3= Thawing pada menit ke-45, dan T4= Thawing pada menit ke-60 menit. Hasil penelitian menunjukkan bahwa thawing pada menit ke-15, 30, 45 dan 60 menit semen beku sapi Simmental berpengaruh sangat nyata (P<0,01) terhadap motilitas dan integritas membran spermatozoa. Rataan nilai motilitas spermatozoa berkisar 31,00% - 42,80%, sedangkan rataan nilai integritas membran berkisar 45,94% - 60,64%. Hasil uji LSD motilitas spermatozoa setelah thawing T1 tidak berbeda nyata dengan T2, T2 berbeda nyata denga T3, T3 berbeda nyata dengan T4. Hasil uji LSD integritas membran setelah thawing T1 berbeda nyata dengan T2, T1 berbeda nyata denga T3, T2 berbeda nyata dengan T3. Berdasarkan uji ortogonal polinomial menunjukkan bahwa pengaruh lama thawing semen beku terhadap persentase motilitas spermatozoa berpola linier dengan persamaan Y= 47,30 – 0,26 (15) = 43,40% dan pengaruh lama thawing semen beku terhadap persentase integritas membran spermatozoa berpola linier dengan persamaan Y= 66,22 – 0,32 (15) = 61,42%.

Kata kunci: Lama thawing, Semen beku, Sapi Simmental, Motilitas dan Integritas membran

Abtrak (Bhs. Inggris)

The aim of this study is to determine the effect of thawing duration of Simmental bulls’ frozen semen on the sperm motility and membrane integrity. The study applies an experimental laboratory with a Randomized Complete Design (RCD). The treatments were T1= Thawing at the 15th minute, T2= Thawing at the 30th minute, T3= Thawing at the 45th minute, and T4= Thawing at the 60th minute. The measured variables are the sperm motility and membrane integrity. The results of this study show that the thawing at the 15th, 30th, 45th and 60th minutes highly significantly affect (P <0.01) the sperm motility and membrane integrity. The mean values of sperm motility ranges from 31.00% to 42.80%, while that of the sperm membrane integrity ranges from 45.94 % to 60.64 %. The LSD test shows that, on the sperm motility, T1 thawing is not different (P<0.05) from T2, T2 is significantly different (P>0.05) from T3, and T3 is significantly different (P>0.05) from T4. While on the sperm membrane integrity, the test also shows that there is a significant difference (P>0.05) between T1 and T2 thawing. T2 is significantly different (P>0.05) from T3, and T3 is significant different (P>0.05) from T4. The orthogonal polynomial test shows that the effect of thawing duration on the percentage of sperm motility of the Simmental bulls’ frozen semen figures a linear equation Y= 47.30 - 0.26 ( 15 ) = 43.40 % and the effect of thawing duration on the percentage of the sperm membrane integrity figures a linear equation Y= 66.22 – 0.32 (15) = 61.42%.

Keywords: Thawing, Frozen Semen, Simmental Bull, Motility, and Membrane Integrity

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