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MAR'ATUN SOLIHAH
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SUPLEMENTASI EKSTRAK DAUN SIRIH MERAH (Piper crocatum) PADA PAKAN SAPI PERAH TERHADAP PRODUKSI GAS TOTAL DAN GAS METAN (CH4) SECARA IN VITRO
Abstrak (Bhs. Indonesia)
Penelitian ini bertujuan untuk mengkaji pengaruh dan efisiensi suplementasi ekstrak daun sirih merah (Piper crocatum) dalam pakan sapi perah terhadap produksi gas total dan gas metan (CH4) secara in vitro. Materi yang digunakan dalam penelitian ini adalah pakan basal sapi perah yang terdiri atas rumput gajah 70% dan konsentrat 30% dengan kandungan protein kasar (PK) 13,38%; serat kasar (SK) 23,27% dan Total Digestible Nutrient (TDN) 62,21%, cairan rumen sapi perah bangsa peranakan Friesian Holstein, ekstrak daun Sirih Merah (Piper crocatum), seperangkat alat perhitungan gas total dan gas metan (CH4) secara in vitro. Metode penelitian adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL). Perlakuan yang uji cobakan yaitu R1: Pakan Basal, R2: R1 + 15 ppm ekstrak daun sirih merah (Piper crocatum), R3: R1 + 30 ppm ekstrak daun sirih merah (Piper crocatum), R4: R1 + 45 ppm ekstrak daun sirih merah (Piper crocatum), R5: R1 + 60 ppm ekstrak daun sirih merah (Piper crocatum). Data dianalisis menggunakan analisis variansi dan dilanjutkan uji Duncan’s Multiple Range Test dan uji Ortogonal Polynomial. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh terhadap penurunan produksi gas total dan gas metan (P<0,01). Hasil uji Duncan’s Multiple Range Test menunjukkan perlakuan R2 dan R5 yang terbaik dengan pemberian ekstrak sirih merah (Piper crocatum) 15 ppm dan 60 ppm mampu menurunkan gas total dan perlakuan R2 yang terbaik dengan pemberian ekstrak sirih merah (Piper crocatum )15 ppm mampu menurunkan gas metan. Hasil Uji Ortogonal Polynomial menunjukkan pengaruh perlakuan produksi gas total dan gas metan berbentuk kubik, dengan suplementasi ekstrak daun sirih merah (Piper crocatum) optimum pada gas total dan gas metan pada level 15 ppm. Hubungan gas total dan gas metan didapat koefisien determinasi 91,08% menunjukkan bahwa hubungan gas total dan gas metan sangat erat. Berdasarkan titik puncak produksi gas total dan gas metan dengan waktu fermentasi 48 jam menunjukkan jam 21 adalah titik puncak produksi gas total dan gas metan.
Abtrak (Bhs. Inggris)
This study aims to assess the impact and the efficiency of supplementation of red betel leaf (Piper crocatum) extract in dairy cattle feed on the production of total gas and methane (CH4) by in vitro. The research materials were basal feed of dairy cattle consisting of 70% forage and 30% concentrate with 13.38% crude protein (CP) content; 23.27% crude fiber (CF) content, and 62.21% Total Digestible Nutrient (TDN), rumen fluid of cross bred Friesian Holstein dairy cattle, red betel leaf (Piper crocatum) extract, and a set of tools to measure the total gas and methane production (CH4) by in vitro. The research method was experimental by using a completely randomized design (CRD). The treatments tested were R1: Basal Feed, R2: R1 + 15 ppm of red betel leaf (Piper crocatum) extract, R3: R1 + 30 ppm of red betel leaf (Piper crocatum) extract, R4: R1 + 45 ppm of red betel leaf (Piper crocatum) extract, R5: R1 + 60 ppm of red betel leaf (Piper crocatum) extract. The data was analyzed by using an analysis of variance and was continued with Duncan’s Multiple Range Test and Orthogonal Polynomial test. The results showed that the treatments decreased the total gas and methane production (P<0.01). Duncan’s Multiple Range Test showed that both R2 and R5 treatments were the best; giving red betel leaf (Piper crocatum) extract as much as 15 ppm and 60 ppm can decrease total gas. So was R2 treatment, giving red betel leaf (Piper crocatum) extract as much as 15 ppm can decrease methane gas. Orthogonal Polynomial test showed that the effect of treatment on total gas and CH4 gas production was in the form of cubic curve where the optimum supplementation of red betel leaf (Piper crocatum) extrac on the total gas and CH4 gas productions was at 15 ppm. The relationship between total gas and CH4 production which is expressed with a determination coefficient of 91.08% showed that the relationship is very close. Meanwhile, during 48 hours of fermentation, the 21st hour is the peak time of total gas and CH4 gas production.
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