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POTENSI TIGA ISOLAT Bacillus subtilis BI, B298 DAN B315 SEBAGAI PEMACU PERTUMBUHAN TANAMAN SOLANACEAE
Abstrak (Bhs. Indonesia)
ABSTRAK Penelitian bertujuan untuk mengetahui karakter tiga isolat B. subtilis sebagai pemacu pertumbuhan tanaman Solanaceae dan mempelajari respon pertumbuhan tanaman Solanaceae setelah aplikasi tiga isolat B. subtilis. Penelitian dilakukan di Laboratorium Perlindungan Tanaman, Unsoed dan lahan sawah Desa Karangwangkal, Purwokerto, pada bulan Maret sampai Agustus 2015. Penelitian menggunakan Rancangan Acak Kelompok dengan 12 perlakuan dan 3 ulangan yaitu perlakuan CTB: Cabai tanpa Bacillus, CB1: Cabai dengan Isolat B1, CB298: Cabai dengan Isolat B298, CB315: Cabai dengan Isolat B315, TeTB: Terung tanpa Bacillus, TeB1: Terung dengan Isolat B1, TeB298: Terung dengan Isolat B298, TeB315: Terung dengan Isolat B315, TTB: Tomat tanpa Bacillus, TB1: Tomat dengan Isolat B1, TB298: Tomat dengan Isolat B298 dan TB315: Tomat dengan Isolat B315. Pengujian di laboratorium meliputi uji penghasil siderofor, uji penghasil IAA, uji pelarut fosfat, uji penghasil nitrogen, dan uji resistensi antibiotika. Variabel yang diamati meliputi tinggi tanaman, jumlah daun, panjang akar, bobot segar tanaman, bobot kering tanaman, bobot akar segar, bobot akar kering, volume akar. Hasil penelitian menunjukkan isolat B1, B298 dan B315 mampu menghasilkan siderofor, IAA, Nitrogen, melarutkan fosfat dan resisten terhadap antibiotika Rifampycin. Perlakuan isolat B. subtilis mampu meningkatkan tinggi tanaman, jumlah daun, panjang akar, bobot segar dan bobot kering tanaman, bobot segar dan bobot kering akar, volume akar tanaman cabai, terung dan tomat.
Abtrak (Bhs. Inggris)
ABSTRACT This study aims to determine the character of the three isolates of B. subtilis as Solanaceae plant growth promoters and learn studying the Solanaceae plant growth response after application of three isolates of B. subtilis. The study was conducted at the Laboratory of Plant Protection, Unsoed and wetland Village Karangwangkal, Purwokerto, starting in March 2015 until August 2015. This study used a randomized block design with 12 treatments and 3 replications are treated CTB: Pepper without Bacillus, CB1: Pepper with Isolate B1, CB298 : Pepper with Isolate B298, CB315: Pepper with Isolate B315, TeTB: Eggplant without Bacillus, TeB1: Eggplant with Isolate B1, TeB298: Eggplant with Isolate B298, TeB315: Eggplant with Isolate B315, TTB: Tomato without Bacillus, TB1: Tomato with Isolate B1, TB298: Tomatoes with isolates B298 and TB315: Tomatoes with isolates B315. Laboratory test include siderophores producing tested, IAA producing tested, phosphate solubilizing tested, nitrogen producing tested, and antibiotics restistance tested. Variables observed included plant heght, number of leaves, root length, fresh weight of plants, plant dry weight, fresh root weight, dry root weight, root volume. The results showed that isolates B. subtilis B1, B298 and B315 are able to produce siderophores, IAA, Nitrogen, capability of solubilizing phosphate and resistance to antibiotics Rifampycin. The treatment isolates of B. subtilis is able to increase plant height, number of leaves, root length, fresh weight and dry weight of plants, fresh weight and dry weight of roots root volume pepper, eggplant and tomatoes.
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