Update Artikelilmiah: 11174

WINDHA KUSUMANINGTYAS

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Abstrak (Bhs. Indonesia)

ABSTRAK
Chemo mechanical caries removal (CMCR) merupakan cara untuk menghilangkan jaringan karies gigi secara non invasif dengan cara melunakkan jaringan menggunakan bahan kimia, kemudian diikuti dengan tindakan ekskavasi menggunakan instrumen manual. Ekskavasi karies yang dalam mempunyai risiko yang besar untuk menyebabkan perforasi rongga pulpa sehingga bahan CMCR yang digunakan dapat masuk ke dalam pulpa. Evaluasi sitotoksisitas gel papain secara in vitro pada kultur jaringan fibroblas pulpa telah dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian gel papain 2%, 4%, 6%, dan 8% sebagai agen chemo mechanical caries removal (CMCR) terhadap pembentukan odontoblast like cell pada perforasi pulpa. Dua puluh lima ekor tikus wistar dibagi menjadi 5 kelompok. Tikus dianestesi menggunakan ketamin, kemudian gigi molar rahang atas dipreparasi sampai pulpa terbuka dan diberi aplikasi bahan gel papain dan kalsium hidroksida. Tikus dikorbankan pada hari ke 28. Pemeriksaan histopatologi dilakukan untuk mengamati odontoblast like cell. Data jumlah odontoblast like cell dianalisis menggunakan uji statistik one way Anova dilanjutkan dengan uji Post Hoc LSD. Hasil pengamatan menunjukkan bahwa rata-rata jumlah odontoblast like cell pada kelompok kontrol sebanyak 16 sel, gel papain 2% sebanyak 25 sel, gel papain  4% sebanyak 27 sel, gel papain 6% sebanyak 30 sel, dan gel papain 8% sebanyak 34 sel. Simpulan penelitian ini adalah terdapat pengaruh pemberian gel papain 2%, 4%, 6%, dan 8% sebagai  agen chemo mechanical caries removal (CMCR) terhadap pembentukan odontoblast like cell pada perforasi pulpa.

Abtrak (Bhs. Inggris)

ABSTRACT

Chemomechanical caries removal (CMCR) is a non invasive technique eliminating carries tissue based on dissolution chemical agent and followed by excavation using manual instruments. Deep caries excavation has a big risk to cause pulp cavity perforation so that the material of CMCR used can get into the pulp. The cytotoxicity papain gels on fibroblasts pulp tissue culture have been evaluated in vitro. The aim of the study was to determine the effect of 2%, 4%, 6%, and 8% papain gels as chemo mechanical caries removal (CMCR) agent on odontoblast like cell formation in pulp perforation. This study carried on total 25 Wistar rats. The rats were randomly divided into five groups. Ketamin was used to anesthetize the rats, the roofs of the pulp chambers of rats maxillary molars were perforated, and then papain gels and calcium hydroxide were applied into the pulp. Rats were sacrified on 28th day after treatment. Histopathological examination was performed to observe the odontoblast like cells. Statistical tests including one way ANOVA and Post-Hoc LSD used to analyze the data. The results showed that the average number of odontoblast like cells in the control group was 27 cells, 2% papain gel was 25 cells, 4% papain gel was 27 cells, 6% papain gel was 30 cells, and 8% papain gel was 34 cells. In conclusion, there is the effect of giving papain gel 2%, 4%, 6% and 8% as the agent chemomechanical caries removal (CMCR) on the formation of odontoblast like cells in pulp perforation.

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