Update Artikelilmiah: 10522

AYU WIKHA NOVIYANA

NIM

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Abstrak (Bhs. Indonesia)

Brotowali (Tinospora crispa) secara empiris digunakan untuk mengobati gouthiperurisemia. Penelitian ini bertujuan untuk mengetahui kandungan fraksi etil asetat (FEA) batang brotowali berdasarkan profil KLT dan aktivitas FEA sebagai antihiperurisemia pada mencit jantan Balb/C yang diinduksi potassium oksonat berdasarkan persentase penurunan kadar asam urat serum.
Identifikasi kandungan kimia dalam FEA dilakukan dengan metode KLT. Uji aktivitas antihiperurisemia dilakukan dengan mencit jantan Balb/C dibagi menjadi 6 kelompok (n=6) terdiri dari kontrol normal (Na-CMC 0,5%), kontrol negatif (potassium oksonat i.p 250 mg/kgBB), kontrol positif (allopurinol 10 mg/kgBB per oral), serta 3 kelompok perlakuan diberi FEA dosis 50 mg/kgBB, 100 mg/kgBB dan 200 mg/kgBB per oral. Darah diambil dua jam setelah induksi potasium oksonat, kemudian diukur kadar asam uratnya menggunakan spektrofotometer. Kadar asam urat dan data persen penurunan kadar asam urat dianalisis statistika Kolmogorov Smirnov kemudian dilanjutkan ANOVA satu arah dan dilanjutkan uji BNT (data berdistribusi normal). Berdasarkan profil KLT, FEA lebih dominan mengandung flavonoid. Hasil uji aktivitas antihiperurisemia FEA menunjukkan persentase penurunan kadar asam urat kelompok FEA dosis 50 mg/kgBB, 100 mg/kgBB dan 200 mg/kgBB adalah 49,03±23,39%, 52,41±12,44%, dan 170,17±16,67%. Hasil uji statistik menunjukkan FEA dosis 200 mg/kgBB memiliki persentase penurunan kadar asam urat yang berbeda signifikan jika dibandingkan kontrol positif (allopurinol). Hal ini mengindikasikan bahwa FEA dosis 200 mg/kgBB memiliki potensi sebagai antihiperurisemia.

Abtrak (Bhs. Inggris)

Brotowali (Tinospora crispa) empirically used to treat gout. This study aims to determine the content of brotowali ethyl acetate fraction (EAF) by TLC profile and activity of EAF as antihiperurisemia in male mice Balb/C strain induced potassium oksonat based on the percentage decrease in blood serum uric acid levels. Identification of chemical constituents in the FEA was conducted by TLC. Male mice Balb/C were divided into 6 groups (n = 6) consisted of normal controls (Na-CMC 0.5%), negative control (oksonat potassium 250 mg/kg i.p), positive control (allopurinol 10 mg/kg orally), and 3 treatment groups were given EAF dose of 50 mg/kg, 100 mg/kg and 200 mg/kg orally. Blood was taken two hours after induction, and then uric acid levels were measured using a spectrophotometer. Uric acid level and decreasing percentage data were analyzed statistically Kolmogorov Smirnov followed one-way ANOVA and LSD test (normal distribution of data). Based on TLC profile showed EAF more dominant contains flavonoid.The results of activity test antihiperurisemia brotowali ethyl acetate fraction showed that the decreasing percentage of uric acid leves treatment group EAF dose of 50 mg/kg, 100 mg/kg and 200 mg/kg was 49,03±23,39%, 52,41±12,44%, and170,17±16,67%. This data showed the greater dose had greater antyhiperurisemic activity. Statistical analysis showed that the EAF dose of 200 mg/kg has a percentage decrease in uric acid levels were significantly different when compared with the positive control (allopurinol). This indicates that the EAF dose of 200 mg/kg potential as an anti hyperuricemia.

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